麒麟菜粗多糖中多糖与蛋白质结合方式

从海藻麒麟菜中提取的粗多糖含有一定量的蛋白质，采用Sevag法在除麒麟菜粗多糖蛋白质，则蛋白质与多糖以相同的比例减少，粗多糖酸解液的pH调整为11．0后，经凝胶SephadexG—l00层析，能使蛋白质部分分离，而酸性条件无法使之分离，实验结果表明，粗多糖中蛋白质不是以游离的形式存在，可能以离子键方式与多糖分子中的硫酸根聚阴离子相结合，提取麒麟菜多糖和纯化过程中，控制合适的pH条件有利于分离蛋白质。

The Mode of Binding Polysaccharide and Protein Together in Crude Polysaccharide of Eucheume Cottoni

Certain amount of proteins are contained in the crude polysaccharide extracting from the seaweed Eucheume cottoni . In removing proteins from crude polgasccharide of Eucheume cottoni by adopting Sevag method, both proteins and polysaccharide reduce in same proportion. The crude polysaccharide liquid of acidolysis is passed through gel Sephadex G100 chromatography after the value its pH is adjusted to 11.0, partial separation of proteins can be occured but separation of protein can not be occurred in acid medium. As shown by experimental result, protein does not exist freely in crude polysaccharide; proteins may bind in the form of ionic bonds to polyanion of sulfate radicals in polysaccharide molecule. During extracting and purifying polysaccharide from Eucheume cotloni, controlling suitable pH condition is advantageous to the separation of protein.