丛枝菌根真菌的增殖技术比较分析

以三叶草作宿主植物，采用单孢接种和多孢接种．接种摩西球囊霉、地表球囊霉、地球囊霉、缩球囊霉和网状球囊霉，在土培、沙培和液培3种条件下进行增殖．收获后分别测定丛枝着生率、泡囊的数量和产孢量等重要的生产指标．研究结果表明，土培法、沙培法、液培法都可用来生产接种剂．但生产效果存在显著差异．所测定的各个指标在液培条件下都较高．沙培法次之，土培法最低．参试的5个菌种都对三叶草表现出亲和性．其中摩西球囊霉和地表球囊霉各项指标都较高．适于生产菌剂；多孢接种较单孢接种具有显著的数量优势．单孢接种可用于菌种纯化．

Comparing the Propagation Technologies of Arbuscular mycorrhizal Fungi

Taking clove as the host plant and adopting single-spore vaccination and multi-spore vaccination, the author enabled glomus. Mosseae, G.versiforme, G.geosporum, G.constrictum and G.reticulatum to be propagated in soil, sand and liquid respectively; and measured their respective arbuscular infection rate, vesicalar quantity and spore production after harvest. As shown by the study, Arbuscular mycorrhizal Fungi inoculum can be produced under the conditions of soil, sand or liquidation but there existed sygnificant difference in productive effect. all indices were in the highest under liquid cultivation, the next under sand cultivation and the last under soil eultivation; 5 fungal species participating in the test showed affinity to clove, in which G.mosseae and G.versiforme showed preponderance in inoculum production;multi-spore vaccination showed quantitative preponderance in reproduction as compared with single-spore vaccination, but the latter can be used for purifying the fungi.