| 产纤维素酶新菌株的筛选及其产酶特性研究 |
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| 引用本文： | 芦志龙,张穗生,吴仁智,陈东,黄日波. 产纤维素酶新菌株的筛选及其产酶特性研究[J]. 广西科学, 2014, 21(1): 22-27 |
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| 作者姓名： | 芦志龙 张穗生 吴仁智 陈东 黄日波 |
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| 作者单位： | 广西科学院;非粮生物质酶解国家重点实验室;国家非粮生物质能源工程技术研究中心;广西生物质产业化工程院;广西生物炼制重点实验室;广西大学生命科学与技术学院; |
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| 基金项目： | 国家973项目(2010CB736209);国家863项目(2012AA022106,2012AA023202,2013AA050701);国家国际合作项目(2010DFB63590,2011DFA61910);国家科技支撑项目(2011BAD22B01);广西科学研究与技术开发计划项目(桂科合10100019-21,桂科攻1099071,桂科合1140010-15);广西自然科学基金项目(2012GXNSFAA053062);广西科学院基本科研业务费项目(11YJ24SW08)资助 |
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| 摘 要： | 【目的】筛选分离新的纤维素酶产酶菌株,并对其生长特性和产酶特性进行研究。【方法】从堆肥样品中分离得到一株产纤维素酶菌株,定名为CP1,利用16SrRNA序列对比和Biolog微生物鉴定系统进行鉴定。通过测定菌株生长速率确定其最适生长条件。采用CMC发酵培养基进行纤维素酶的研究,确定其产酶曲线。测定粗酶液最适作用温度和pH值,热稳定性和金属离子对其影响。【结果】经鉴定该菌株为梭形芽孢杆菌(Lysinibacillus fusiformis),其最适生长温度为30℃,最适生长pH值为6。在含CMC-Na的培养基培养,该菌株的生长与产酶同步进行,培养48h菌株生长量达到最大,培养液的CMC酶活力同时达到最大值,为0.46U/mL。该菌株所产的纤维素酶既有酸性CMC酶活力,又有碱性CMC酶活力,并以碱性CMC酶为主,酸性CMC酶的活力只有碱性CMC酶的71.4%。其酸性CMC酶的最适作用pH值为6.0,碱性CMC酶的最适作用pH值为8.0。另外,该菌株所产碱性CMC酶活的最适作用温度为40~50℃,而且0.5%的Cu2+使其酶活降低45%。【结论】菌株CP1为首次报道的梭形芽孢杆菌产纤维素酶新菌株,具有独特的酸碱性环境下的纤维素酶活力。
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| 关 键 词： | 梭形芽孢杆菌 纤维素酶 新菌株 CMC酶活力 |
| 收稿时间： | 2013-09-04 |
| 修稿时间： | 2013-10-11 |
Breeding of a Novel Cellulase-producing Bacterial Strain and Characters Analysis of Its Cellulase |
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| LU Zhi-long,ZHANG Sui-sheng,WU Ren-zhi,CHEN Dong and HUANG Ri-bo. Breeding of a Novel Cellulase-producing Bacterial Strain and Characters Analysis of Its Cellulase[J]. Guangxi Sciences, 2014, 21(1): 22-27 |
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| Authors: | LU Zhi-long ZHANG Sui-sheng WU Ren-zhi CHEN Dong HUANG Ri-bo |
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| Affiliation: | Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China,Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;College of Life Science & Technology of Guangxi University, Nanning, Guangxi, 530004, China and Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China |
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| Abstract: | |
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| Keywords: | Lysinibacillus fusiformis cellulase novel strain CMCase activity |
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