Isolation of tomato proteinase inhibitor Ⅱ gene and the function of its intron |
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作者单位: | Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China
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摘 要: | The genomic DNA sequence of tomato proteinase inhibitor Ⅱ gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length of 109 bp, owns typical structures of GT/AG dinucleotides at both ends and high content of AT base pairs which accounts for 80.7% of the total nucleotides. As shown by recombination experiment, the TPI sequence could efficiently promote the expression of the reporter gene gusA and this effect was independent of the position and orientation of the intron, thus showing its role as an enhancer. Such experiments as gel retardation assays, GUS histochemical staining and GUS fluorometric assays further demonstrated that TPI sequence maybe has promoter-like activity.
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关 键 词: | proteinase inhibitor Ⅱ gene |
收稿时间: | 2002-01-11 |
Isolation of tomato proteinase inhibitor Ⅱ gene and the function of its intron |
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Authors: | XIE Xianzhi WU Naihu |
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Institution: | Institute of Developmental Biology, Chinese Academy of Sciences ,Beijing 100080 ,China |
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Abstract: | The genomic DNA sequence of tomato proteinase inhibitor Ⅱ gene (named tin2i, whose accession number in GenBank is AF007240) was isolated by PCR techniques. The intron sequence (TPI), with a length of 109 bp, owns typical structures of GT/AG dinucleotides at both ends and high content of AT base pairs which accounts for 80.7% of the total nucleotides. As shown by recombination experiment, the TPI sequence could efficiently promote the expression of the reporter gene gusA and this effect was independent of the position and orientation of the intron, thus showing its role as an enhancer. Such experiments as gel retardation assays, GUS histochemical staining and GUS fluorometric assays further demonstrated that TPI sequence maybe has promoter-like activity. |
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Keywords: | tomato intron enhancer |
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