| 中药柴胡提取物对人肝癌细胞BEL-7402细胞内游离钙离子浓度的影响 |
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| 引用本文： | 韩晓红,盖晓东,薛延军. 中药柴胡提取物对人肝癌细胞BEL-7402细胞内游离钙离子浓度的影响[J]. 北华大学学报(自然科学版), 2004, 5(4): 323-325 |
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| 作者姓名： | 韩晓红 盖晓东 薛延军 |
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| 作者单位： | 北华大学,医学院,吉林,吉林,132013;第四军医大学,吉林军医学院,吉林,吉林,132013 |
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| 摘 要： | 目的测定中药柴胡(Bupleurun Chinese DC, BCDC)提取物对人肝癌细胞BEL-7402细胞内游离钙离子浓度([Ca2 ]i)的影响,以探索柴胡逆转BEL-7402细胞多药耐药的机制.方法以Fura-2/AM为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计分别测定不同条件下的BEL-7402细胞内[Ca2 ]i.结果柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降(P<0.001).结论柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降,为一种钙离子通道阻滞剂(Calcium channel blocker, CCB),提示钙离子通道阻滞作用为柴胡逆转BEL-7402细胞多药耐药的机制之一.
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| 关 键 词： | 柴胡 细胞内游离钙 Fura-2/AM BEL-7402 |
| 文章编号： | 1009-4822(2004)04-0323-03 |
| 修稿时间： | 2003-12-12 |
Effect of the Extracts from BCDC on Intracelluar Free Calcium of Human Hepatoma BEL-7402 Cells |
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| HAN Xiao-hong,GAI Xiao-dong,XUE Yan-jun. Effect of the Extracts from BCDC on Intracelluar Free Calcium of Human Hepatoma BEL-7402 Cells[J]. Journal of Beihua University(Natural Science), 2004, 5(4): 323-325 |
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| Authors: | HAN Xiao-hong GAI Xiao-dong XUE Yan-jun |
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| Affiliation: | HAN Xiao-hong~1,GAI Xiao-dong~1,XUE Yan-jun~2 |
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| Abstract: | ObjectiveTo measure the effect of the extracts from BCDC on intracellular free calcium of human hepatoma BEL-7402 cells, in order to explore the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma.MethodsDouble wavelength spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular free calcium(i) was used to measure the changes of i in BEL-7402 cells of human hepatoma affected by BCDC.ResultsBCDC can reduce intracellular free calcium of human hepatoma BEL-7402 cells (P<(0.001)).ConclusionBCDC is one of calcium channel blocker, which indicate that the effect of calcium channel blocker may be one of the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma. |
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| Keywords: | Bupleurun Chinese DC Intracellular free calcium Fura-2/AM BEL-7402 |
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