| 加工番茄多聚半乳糖醛酸酶(PG)基因的cDNA克隆 |
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| 引用本文： | 李惠,肖璐,祝建波,崔百明,乐锦华,曹连莆.加工番茄多聚半乳糖醛酸酶(PG)基因的cDNA克隆[J].石河子大学学报,2000,4(3):220-222,252. |
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| 作者姓名： | 李惠 肖璐 祝建波 崔百明 乐锦华 曹连莆 |
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| 作者单位： | [1]石河子大学农学院农业科学系 [2]石河子大学生物科学系 |
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| 摘 要： | 以加工番茄８７－５为材料，运用反转录ＰＣＲ（ＲＴ－ＰＣＲ）技术成功地将ＰＧ基因的ｃＤＮＡ序列克隆到ｐＧＥＭ－Ｔ载体上，并利用酶切及ＰＣＲ进行了初步鉴定。
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| 关 键 词： | RT－PCR cDNA PG基因 克隆 番茄 转基因育种 |
| 文章编号： | 1007-7383(2000)03-0220-13 |
Clonging the cDNA of Polygaacturonase in Processing Tomato |
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| LI Hui\ ,\ XIAO Lu\ ,\ ZHU Jian bo\ ,\ CUI Bai ming\ ,\ LE Jin hua\ ,\ CAO Lian pu\.Clonging the cDNA of Polygaacturonase in Processing Tomato[J].Journal of Shihezi University(Natural Science),2000,4(3):220-222,252. |
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| Authors: | LI Hui\ \ XIAO Lu\ \ ZHU Jian bo\ \ CUI Bai ming\ \ LE Jin hua\ \ CAO Lian pu\ |
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| Institution: | LI Hui\ 1,\ XIAO Lu\ 2,\ ZHU Jian bo\ 2,\ CUI Bai ming\ 2,\ LE Jin hua\ 2,\ CAO Lian pu\ 1 |
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| Abstract: | The entire cDNA encoding polygalacturonase was successfully cloned with RT PCR technique from pericarp of ripe processing tomato fruits 87 5,and its whole nucleotide sequence was tentatively analysed and identified. |
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| Keywords: | cDNA encoding polygalacturonase RT PCR PG cDNA clone |
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