| 正交设计－流动注射荧光光谱分析法研究：过氧化物酶测定体系选择和反应机理 |
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| 引用本文： | 彭学军,李庆阁,许金钩,陈国珍.正交设计－流动注射荧光光谱分析法研究：过氧化物酶测定体系选择和反应机理[J].厦门大学学报(自然科学版),1995(3). |
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| 作者姓名： | 彭学军 李庆阁 许金钩 陈国珍 |
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| 摘 要： | 采用正交设计－流动注射荧光光谱分析法。对测定过氧化物酶体系的各影响因素进行了正交直观分析和方差分折，结果表明：对羟基苯丙酸是影响反应的显著性因素：十六烷基三甲基溴化铵对反应有增敏作用，并对其增敏原因进行了探讨；提出一种过氧化物酶催化下的反应机理。
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| 关 键 词： | 正交设计，流动注射，荧光光谱分析，过氧化物酶 |
Studies on Experimental Paramenters and Reaction Mcchanism in Peroxidase Detection by Orthogonal Design-Flow Injection Fluorescence Spectrornetry |
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| Peng Xuejun, Li Qingge,Xu Jingou,Chen Guozhen.Studies on Experimental Paramenters and Reaction Mcchanism in Peroxidase Detection by Orthogonal Design-Flow Injection Fluorescence Spectrornetry[J].Journal of Xiamen University(Natural Science),1995(3). |
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| Authors: | Peng Xuejun Li Qingge Xu Jingou Chen Guozhen |
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| Institution: | Dept.of Chem. |
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| Abstract: | The influence of experimental parameters,H_2O_2,HPPA and CTAB on thedetection of peroxidase was studied by Orthogonal Design-Flow Injection Fluorescence Spectrom-etry.HPPA was found to be the distinguished factor to affect the detection of peroxidase. CTABcan enhance the signal intensity,and the reseaons were also discussed.A probable reactionmechanism was suggested for the formation of fluorescence products with coupling of radical. |
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| Keywords: | Orthogonal design Flow injection Fluorescence spectrometry Peroxi-dase |
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