| 新生大鼠海马神经干细胞的体外培养及鉴定 |
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| 引用本文： | 张建平,司银楚,刘大全.新生大鼠海马神经干细胞的体外培养及鉴定[J].井冈山学院学报,2009,30(2):75-77. |
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| 作者姓名： | 张建平 司银楚 刘大全 |
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| 作者单位： | 张建平,刘大全,ZHANG Jian-ping,Liu Da-quan(井冈山大学,医学院,江西,吉安,343009);司银楚,SI Yin-chu(北京中医药大学,基础医学院,北京,100029)
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| 摘 要： | 的探讨新生大鼠海马神经干细胞（NSC）的体外培养和诱导分化的条件和特点。方法分离出生1d大鼠海马，在表皮生长因子、碱性成纤维生长因子和B27联合作用下使其稳定增殖，用5-溴脱氧尿苷（BMU）标记处于增殖状态的神经干细胞，应用免疫荧光染色方法行巢蛋白（Nestin）、5-溴脱氧尿苷（BrdU）、β-Ⅲ型微管蛋白（Tuj-1）、波形蛋白（Vimenfin）和Galc-C免疫荧光染色，对NSC的增殖及其分化的细胞进行鉴定。结果体外培养的NSC增殖成神经干细胞球并传代，鉴定为Nestin染色阳性细胞和5-溴脱氧尿苷（BrdU）标记染色阳性细胞，并可诱导分化为神经元细胞（Tuj-1染色阳性细胞）、神经胶质细胞（Vimentin染色阳性细胞）和少突胶质细胞（GMc-C染色阳性细胞）。结论采用无血清培养基中加入特定生长因子的培养技术，可培养出在体外稳定增殖并有多向分化潜能的新生大鼠海马神经干细胞。
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| 关 键 词： | 新生大鼠 神经干细胞 细胞增殖 细胞分化 |
Culture and identification of Neonate Rat hippocampus Neural Stem Cells |
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| ZHANG Jian-ping,SI Yin-chu,Liu Da-quan.Culture and identification of Neonate Rat hippocampus Neural Stem Cells[J].Journal of Jinggangshan University,2009,30(2):75-77. |
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| Authors: | ZHANG Jian-ping SI Yin-chu Liu Da-quan |
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| Institution: | ZHANG Jian-ping1,SI Yin-chu2,Liu Da-quan1 |
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| Abstract: | Objective: To explore the conditions and characteristics of culture and induced differentiation of neonate rat hippocampus neural stem cells in vitro.Methods: The cells from hippocampus of neonatal rats(1 day)were mechanically dissociated and proliferated steadily under the combined effect of epidermal growth factor(EGF),basic fibroblast growth factor(bFGF)and B27.The rat neural stem cells in different pro-liferative status were marked by 5-bromodeoxyuridine(BrdU).Then the proliferation and differentiation ... |
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| Keywords: | Neonate Rat Neural Stem Cells cell proliferation differentiation |
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