首页 | 本学科首页   官方微博 | 高级检索  
     检索      

水溶性壳聚糖的制备及其体外抗菌活性
引用本文:李再新,周健,王川,李曾维,黄福,唐盛兰.水溶性壳聚糖的制备及其体外抗菌活性[J].四川理工学院学报(自然科学版),2005,18(1):79-82.
作者姓名:李再新  周健  王川  李曾维  黄福  唐盛兰
作者单位:四川理工学院生物工程系,四川,自贡,643000
基金项目:四川省教育厅基金资助项目
摘    要:设计中温长时间歇浸泡新工艺,以龙虾虾壳为原料,制备壳聚糖;比较壳聚糖降解反应条件的影响,制备水溶性壳聚糖并开展其抗菌试验。利用该工艺方法制得了低水分和灰分、高粘度、高产量、脱乙酰度达90.17±0.03%壳聚糖;在80℃、2h和3%双氧水浓度作用下,制得产率达77.20±0.02%的水溶性壳聚糖,且该水溶性壳聚糖显著抑制大肠杆菌和枯草杆菌的生长,尤其对枯草杆菌的抑菌效果更为显著(p<0.01)。实验表明利用设计的新方法能获得高效率、高质量和具有显著抗菌作用的水溶性壳聚糖,并为壳聚糖的深入研究和开发奠定一定的理论和物质基础。

关 键 词:壳聚糖  水溶性壳聚糖  抗菌
文章编号:1673-1549(2005)01-0079-04
修稿时间:2004年11月25

Preparation of Water-soluble Chitosan and Its Antibacterial Activity in Vitro
LI Zai-xin,ZHOU Jian,WANG Chuan,LI Zeng-wei,HUANG Fu,TANG Sheng-lan.Preparation of Water-soluble Chitosan and Its Antibacterial Activity in Vitro[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2005,18(1):79-82.
Authors:LI Zai-xin  ZHOU Jian  WANG Chuan  LI Zeng-wei  HUANG Fu  TANG Sheng-lan
Abstract:A chitosan is manufactured from the crust of lobster by the method of the long-time, middling temperature and intermission in the experiment. And the water-soluble chitosan is produced in different time, different temperature and different concentration of H2O2, according to the effect of various factors on the quality of water-soluble chitosan in the hydrolyzation of chitosan. The chitosan confers the low moisture and remains of burned chitosan, the high percentage of deacetylated chitosan, the extreme viscousness (90.17± 0.03%). The water-soluble chitosan, a rate of 77.20±0.02%, shows in the condition of 80℃, 2h and 3% H2O2, a nd inhibits growth of two bacterium, Escherichia coli(G-) and Bacillus subtilis(G+), especially Bacillus subtilis(G+)(p<0.01). The water-soluble chitosan of high qulity produced efficiently by the innovated methods has the significence of antibacterial activity. The findings indicate that the further investigation and development of water-soluble chitosan is needed.
Keywords:chitosan  water-soluble chitosan  antibacterium
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号