Solubilization of prolactin receptor by a Zwitterionic detergent |
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Authors: | W. R. Church K. E. Ebner |
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Affiliation: | (1) Department of Biochemistry, University of Kansas Medical Center, 66103 Kansas City, Kansas, USA |
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Abstract: | Summary About 60% of the prolactin receptors were solubilized from rabbit mammary gland membranes by Zwittergent 3–12. The use of Zwittergent 3–12 resulted in increased sensitivity of the receptor assay and permitted use of ovine prolactin instead of human growth hormone in the receptor assay.Acknowledgments. This work was supported by grant AM 17476 from the National Institutes of Health. |
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