| 钒酸钠和氯化锂对盐藻质膜排钠系统的影响 |
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| 引用本文： | 魏亮,邓婷婷,刘谊,梁雪,易芬芬,曹毅,乔代蓉. 钒酸钠和氯化锂对盐藻质膜排钠系统的影响[J]. 四川大学学报(自然科学版), 2006, 43(3): 677-681 |
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| 作者姓名： | 魏亮 邓婷婷 刘谊 梁雪 易芬芬 曹毅 乔代蓉 |
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| 作者单位： | 四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065;四川大学生命科学学院,四川省生物信息与代谢工程共享实验平台,成都,610065 |
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| 摘 要： | 杜氏盐藻(Dunaliella salina)有着极强的耐盐碱性.盐藻在低pH值条件下,主要依靠质膜上的Na+/H+逆向转运蛋白实现Na+的外排.在pH值较高的环境中,由于Na+/H+逆向转运蛋白不能够很好的发挥效能,所以盐藻质膜上很可能存在着不依赖于跨膜质子电化学势梯度的排钠机制.通过将盐藻培养液的pH值稳定在7.1和8.9,比较Na+/H+逆向转运蛋白的特异性抑制剂LiCl,以及P型ATPase的特异性抑制剂钒酸钠对盐藻生长的影响,推测盐藻质膜上存在着由不依赖于跨膜质子电化学势梯度的P型ATPase所介导的排钠机制.
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| 关 键 词： | 杜氏盐藻 钒酸钠 氯化锂 排钠机制 |
| 文章编号： | 0490-6756（2006）03-0677-05 |
| 收稿时间： | 2005-10-18 |
| 修稿时间： | 2005-10-18 |
The Effects on Na~(+)-export Mechanisms in Plasma Membrane of Dunaliella salina Caused by LiCl and Orthvanadate |
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| WEI Liang,DENG Ting-ting,LIU Yi,LIANG Xue,YI Fen-fen,CAO Yi,QIAO Dai-rong. The Effects on Na~(+)-export Mechanisms in Plasma Membrane of Dunaliella salina Caused by LiCl and Orthvanadate[J]. Journal of Sichuan University (Natural Science Edition), 2006, 43(3): 677-681 |
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| Authors: | WEI Liang DENG Ting-ting LIU Yi LIANG Xue YI Fen-fen CAO Yi QIAO Dai-rong |
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| Affiliation: | College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education,College of Life Science; Sichuan University; Sichuan Public Experiment Platform of Bioinformatics and Metabolic Engineering; Key Laboratory of Bio-resources and Eco-environment Ministry of Education |
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| Abstract: | The unicellular marine alga Dunaliella salina can grow in media of both high salinity and high alkalinity.At low pH,active Na~(+)-export is carried out by means of a plasma membrane localized Na~(+)/H~(+)antiporter.At high alkalinity,the Na~(+)/H~(+)antiporter is inefficient.For these reasons,in the plasmamembrane of the D.salina,there may be other Na~(+)-export systems which are not dependent on the electrochemical gradient generated by the plasma membrane H~(+)-ATPase.In this paper,the alga was cultured i... |
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| Keywords: | Dunaliella salina orthvanadate LiCl Na~(+)-export mechanism |
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