| NO诱导血管平滑肌细胞凋亡中Ca~(2 )的作用 |
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| 引用本文： | 田雪梅,马晓东. NO诱导血管平滑肌细胞凋亡中Ca~(2 )的作用[J]. 中山大学学报(自然科学版), 2001, 0(1) |
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| 作者姓名： | 田雪梅 马晓东 |
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| 作者单位： | 中山大学化学与化学工程学院 广东广州510275(田雪梅)，第一军医大学中心实验室 广东广州510515(马晓东) |
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| 摘 要： | 探讨了ＮＯ诱导血管平滑肌细胞凋亡与细胞内游离Ｃａ２＋之间的关系．通过粘附式细胞仪和Ｃａ２＋ 荧光探针Ｆｌｕｏ－３／ＡＭ，检测分析了ＮＯ在供体ＳＮＡＰ的作用下，血管平滑肌细胞中游离Ｃａ２＋浓度的变化； 又通过ＳＮＡＰ与维拉帕米、ＥＧＴＡ、肝素钠、普鲁卡因共同孵育的方法，观测了Ｃａ２＋浓度变化在细胞凋亡中 的作用．得出ＳＮＡＰ能使细胞中游离Ｃａ２＋浓度升高，而胞外Ｃａ２＋内流在其中起主要作用；并且阻断胞外 Ｃａ２＋内流能够抑制ＳＮＡＰ所诱导的血管平滑肌细胞的凋亡．提示了胞内Ｃａ２＋浓度升高可能是ＳＮＡＰ诱导血管 平滑肌细胞凋亡的一条途径．
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| 关 键 词： | NO 血管平滑肌细胞 Ca~(2 ) |
Effect of Intracellular Ca~(2 ) on Apoptosis in Vascular Smooth Muscle Cells Induced by NO |
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| TIAN Xue-mei,MA Xiao-dong. Effect of Intracellular Ca~(2 ) on Apoptosis in Vascular Smooth Muscle Cells Induced by NO[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 0(1) |
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| Authors: | TIAN Xue-mei MA Xiao-dong |
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| Abstract: | To investigate the relationship between the concentration of intracellular Ca2 and apoptosis in vascular smooth muscle cells (VSMC) induced by SNAP, one of NO donors, the concentration change of Ca2 in VSMC caused by SNAP was deteined. Verapamil, EGTA, procanine and sodium heparin were also used to investigate the effects of Ca2 on the apoptosis of VSMC induced by SNAP. The results showed that SNAP increased intracellular Ca2 concentration in VSMC and thsi is ascribed to the extraeellular Ca2 influx. The apoptosis was inhibited by blocking influx of extracellular Ca2 . The results suggested that the increase in intracellular Ca2 through influx of extraeellular Ca2 induced by SNAP may play a role in apoptosis of vascular smooth muscle cells induced by SNAP. |
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| Keywords: | nitric oxide Ca2 vascular smooth muscle cells |
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