固定化金属离子亲和色谱研究进展

 固定化金属离子亲和色谱是将过渡金属离子通过配体螯合在固相基质上，通过过渡金属离子与靶分子的组氨酸或半胱氨酸特异性结合形成相对稳定的复合物，最后以竞争性洗脱方式实现靶分子的富集与纯化，其核心为金属螯合亲和基质材料制备。具有亲和选择性高、生物兼容性好、可逆再生等优势，迄今已发展了40余年，广泛应用于靶分子的特异性富集、分离与纯化，本文综述了近3年固定化金属离子亲和色谱纳米材料、微球色谱基质、棉纤维、分子印迹材料、整体材料、共价有机骨架等方面的研究进展。

Recent advances in immobilized metal ion affinity chromatography

The immobilized metal ion affinity chromatography (IMAC) is based on the affinity between the transition metal ions and the target molecules, with the transition metal ions being immobilized on the solid matrix by ligands and combined with histidine or cysteine of the target molecules to form relatively stable complexes. The enrichment and the purification of the target molecules are achieved by the competitive elution. The IMAC, with the preparation of the metal chelate affinity materials as its core technology, has been developed for more than 40 years and widely used in the specific enrichment, separation and purification of the target molecules due to its high affinity selectivity, good biocompatibility and reversible regeneration. From the point of view of the analytical chemistry, the recent three-year advances in the IMAC nanomaterials, microsphere chromatography matrices, cotton fibers, molecularly imprinted materials, monolith and covalent organic frameworks are reviewed in this paper.