熊果苷纳米粒的制备及用于烧伤皮肤修复研究

通过以牛血清白蛋白V(BSA)为载体，去溶剂化法制备熊果苷纳米粒(Arb-BSA-NP)，研究其对大鼠烧伤修复的效果. 利用粒径分布和Zeta电位对纳米粒进行表征，结果显示:熊果苷与白蛋白比例1 ∶ 10、pH7.0的条件下用戊二醛交联12 h，粒子大小均一且稳定性较好，平均粒径为175.26±11.04 nm，Zeta电位为-29.12±0.80 mV；将细菌纤维素膜浸渍于含Arb-BSA-NP纳米粒溶液中获得负载熊果苷纳米粒的纤维膜敷料(Arb-BSA-BC)，并对该材料进行SEM分析，显示Arb-BSA-NP纳米颗粒分布在材料表面；通过SD大鼠烧伤模型动物实验评价该敷料对烧伤创面愈合的效果，结果显示负载熊果苷纳米粒的纤维膜敷料有助于烧伤创面的恢复和愈合.

The Preparation of Arb-BSA-NP Nanoparticals and Their Application to Burn Injury Repair

Bovine serum albumin V(BSA) was used as the carrier to prepare arbutin(Arb)-loaded nanoparticles (Arb-BSA-NP)with the desolvation method and the curative effect of Arb-BSA-NP on rat burn injury was studied. The particle size distribution and Zeta potential analysis were performed to characterize Arb-BSA-NP, and the results showed that the average particle size of Arb-BSA-NP was 175.26±11.04 nm and the average Zeta potential was -29.12±0.80 mV, which suggested that Arb-BSA-NP had a uniform particle size and good stability after 12 h of glutaraldehyde crosslinking under the conditions of pH7.0 and the ratio of arbutin to albumin was 1 : 10. Cellulose membrane was then immersed into the solution containing Arb-BSA-NPs, and the nanoparticles were precipitated onto the surface to form Arb-BSA-BC, which were manifested with the SEM analysis. The curative effect of Arb-BSA-BC on rat burn injury was attested with the burn repair experiment. It was proved that Arb-BSA-BC contributed to the repair and healing of burn injury.