拟南芥BLI基因调控植物衰老的研究

BLISTER(BLI)蛋白是多种应激反应的调节因子,可以促进植物对环境的适应性.BLI蛋白通过抑制IRE 1蛋白以维持植物的正常生长,缺失BLI蛋白的拟南芥植株出现多种发育缺陷表型.文章以拟南芥bli突变体为实验材料,通过细胞生物学及遗传学方法检测其衰老相关表征,发现bli突变体植株早期出现叶片黄化、叶绿素含量下降、...

Regulation of Plant Senescence by BLI in Arabidopsis thaliana

As a regulator of various stress responses, protein BLI promotes the adaptability of plants to the environment and maintains the normal growth of plants by inhibiting protein IRE1. Arabidopsis thaliana individuals without protein BLI are shown to have the mutant phenotype of pleiotropic development. The senescence-related characteristics of BLI mutant Arabidopsis thaliana plants were studied with the cell biology and genetic methods. It was found that a series of senescence phenotypes, such as leaf yellowing, decreased chlorophyll content and increased cell conductivity, appeared in the early stage of the BLI mutant plants and, at the same time, the expression of senescence-related genes in BLI mutant plants was up-regulated and the expression of photosynthesis-related genes was down-regulated. These results preliminarily proved that protein BLI was involved in plant senescence. Applying exogenous ROS to wild-type Col-0 plants showed that the BLI expression was reduced and GUS staining showed that BLI was expressed in all parts of the plant, which provides clues for further analyzing the molecular network for BLI regulating senescence.