| 多重PCR技术分析日本对虾Marsupenaeus japonicus精巢和卵巢差异表达基因 |
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| 引用本文： | 王艺磊 贾锡伟 邹志华 张子平. 多重PCR技术分析日本对虾Marsupenaeus japonicus精巢和卵巢差异表达基因[J]. 科学技术与工程, 2004, 4(5): 374-379 |
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| 作者姓名： | 王艺磊 贾锡伟 邹志华 张子平 |
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| 作者单位： | 集美大学,水产生物技术研究所,水产学校,夏门,361021;美国密歇根州立大学水产与野生生物系,密歇根,美国 |
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| 基金项目： | 国家自然科学基金 ( 3 0 0 70 5 97)，教育部骨干教师基金资助 |
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| 摘 要： | 通过多重PCR方法，对采用SSH(抑制消减杂交)技术制备日本对虾卵巢特异探针并筛选卵巢cDNA全长文库所获得的8个阳性克隆进行分析，研究这些新克隆的基因在精巢和卵巢的差异表达情况。这8个基因可分为二类，一类为卵巢特异表达的基因，一类为卵巢的表达量高于精巢的差异表达基因。
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| 关 键 词： | 日本对虾 精巢 卵巢 多重PCR 基因表达 |
| 文章编号： | 1671-1815(2004)05-0374-06 |
| 修稿时间： | 2004-01-06 |
Detection of Differential Expression Genes of Testies and Ovary in Marsupenaeus Japonicus Using Multiplex PCR Technique |
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| WANG Yilei,JIA Xiwei,ZOU Zhihu,ZHANG Ziping. Detection of Differential Expression Genes of Testies and Ovary in Marsupenaeus Japonicus Using Multiplex PCR Technique[J]. Science Technology and Engineering, 2004, 4(5): 374-379 |
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| Authors: | WANG Yilei JIA Xiwei ZOU Zhihu ZHANG Ziping |
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| Abstract: | A shrimp ovary cDNA library was constructed by SMART method and this library was screened using the enriched ovary cDNA probes prepared with SSH which the testis cDNA was used as the driver. Eight genes which may have a higher expression level in the ovary compared with testis have been cloned. To investigate the mRNA levels of eight genes in ovary and testis, using multiplex PCR, actin gene was used as a internal gene to detect differential expression genes of testis and ovary. The results show that the eight genes can be divided into two types, one type is a specific gene in ovary, another type is differential expression genes which have a higher expression level in the ovary. |
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| Keywords: | Marsupenaeus japonicus testis ovary multiplex PCR gene expression |
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