| 基于mtDNA COI基因序列的盐肤木种群遗传变异 |
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| 引用本文： | 安淼,马恩波,任竹梅. 基于mtDNA COI基因序列的盐肤木种群遗传变异[J]. 山西大学学报(自然科学版), 2011, 34(1): 127-130 |
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| 作者姓名： | 安淼 马恩波 任竹梅 |
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| 作者单位： | 1. 山西大学,生命科学学院,山西,太原,030006
2. 山西大学,应用生物学研究所,山西,太原,030006 |
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| 基金项目： | 国家自然科学基金(30670361); 山西省自然科学基金(2007011078); 山西省回国留学人员科研资助项目 |
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| 摘 要： | 基于mtDNA COI基因序列对贵州、湖北和四川的盐肤木(Rhus chinensis Mill.)种群进行遗传变异分析.研究共测得盐肤木9个种群50个个体的mtDNA COI基因长度为1.37kb的序列,在测得的序列中有3个核苷酸位点存在变异(占所测核苷酸序列的0.22%),T、C、A、G 4种核苷酸的组成分别为34.1%、21.8%、22.5%、21.6%,其中A+T含量(56.6%)高于G+C含量(43.4%).共检测到3个单倍型(HapA、HapB和HapC),HapA出现在所有种群中,是出现频率最高的单倍型,占所测序列的90%,该单倍型可能是祖先单倍型;HapB由安县的1个个体和竹山的3个个体共享,而HapC只存在于榕江的1个个体.分析表明盐肤木种群具低水平单倍型多样性(0.000 2)和核苷酸多样性(0.187 0),基于该基因序列的盐肤木种群间遗传多样性和遗传分化均较小.
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| 关 键 词： | 盐肤木 mtDNA COI基因序列 遗传变异 |
Genetic Variation of Rhus chinensis Populations Based on mtDNA COI Sequences |
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| AN Miao,MA En-bo,REN Zhu-mei. Genetic Variation of Rhus chinensis Populations Based on mtDNA COI Sequences[J]. Journal of Shanxi University (Natural Science Edit, 2011, 34(1): 127-130 |
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| Authors: | AN Miao MA En-bo REN Zhu-mei |
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| Affiliation: | AN Miao1,MA En-bo2,REN Zhu-mei1(1.School of Life Science,Shanxi University,Taiyuan 030006,China,2.Research Institute of Applied Biology,China) |
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| Abstract: | Mitochondrial DNA(mtDNA) cytochrome c oxidase I(COI) gene was used to exmaine the genetic structure and diversity of a Rhus chinensis populations from China.The sequences(1.37 kb in length) were obtained from 50 individuals of the 9 R.chinensis populations.In all the sequences,3 nucleotide site substitutions and 3 haplotypes(HapA,HapB and HapC) were examined,respectively.The base compositions were calculated and the results were:T,34.1%;C,21.8%;A,22.5% and G 21.6%,respectively.The average A + T content was ... |
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| Keywords: | Rhus chinensis mtDNA COI gene sequence genetic diversity |
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