| 大叶白麻组织培养再生苗有关酶的初步研究 |
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| 引用本文： | 张煜星,魏凌基,黄文华,陈季恂,马兵刚. 大叶白麻组织培养再生苗有关酶的初步研究[J]. 石河子大学学报(自然科学版), 1993, 0(3) |
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| 作者姓名： | 张煜星 魏凌基 黄文华 陈季恂 马兵刚 |
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| 作者单位： | 石河子农学院生物基础部(张煜星，魏凌基，黄文华，陈季恂)，石河子农学院生物基础部(马兵刚) |
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| 摘 要： | 采用聚丙烯酸胺凝胶垂直板电泳技术,对大叶白麻实生苗、愈伤组织、组织培养再生苗进行了过氧化物酶同工酶酶谱分析。再生苗过氧化物酶谱带与实生苗基本相似。但是,组织培养再生苗的POD、CAT活性明显高于实生苗,而且脂质过氧化的产物MDA含量也明显低于实生苗。
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| 关 键 词： | 再生苗 愈伤组织 过氧化氢酶 过氧化物酶同工酶 大叶白麻 |
A Preliminary Study on Related Enzymes in Regeneration Seedling from Poacynum Tissue Culture |
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| Zhang Yuxing Wei Linji Huang Wenhua Chen Jixun Ma Binggang. A Preliminary Study on Related Enzymes in Regeneration Seedling from Poacynum Tissue Culture[J]. Journal of Shihezi University(Natural Science), 1993, 0(3) |
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| Authors: | Zhang Yuxing Wei Linji Huang Wenhua Chen Jixun Ma Binggang |
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| Affiliation: | Dept. of Biology |
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| Abstract: | Peroxidase isozyme patterns in seedling, callus, and regeneration seedling from tissue culture of Poacynum were analyzed by vertical polyacrylamide gel electrophoresis. The isozyme zymograms of peroxidase of the regeneration seedling were similar to those of the control seedling.The activities of catalase and peroxidase increased, the lipoid peroxidation decreased and the mal-ondialdehyde content significantly decreased in regeneration seedling from tissue culture of Poacynum. |
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| Keywords: | regeneration-seedling callus catalase peroxidase-isozyme Poacynum hender-sonii |
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