| 透明颤菌血红蛋白基因在苏云金杆菌中的表达 |
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| 引用本文： | 周艳芬,赵晓瑜,张玉,冯书亮,王容燕. 透明颤菌血红蛋白基因在苏云金杆菌中的表达[J]. 河北大学学报(自然科学版), 2006, 26(1): 33-37 |
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| 作者姓名： | 周艳芬 赵晓瑜 张玉 冯书亮 王容燕 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001;河北大学,生命科学学院,河北,保定,071002;河北大学,生命科学学院,河北,保定,071002;河北省农林科学院,植物保护研究所,河北,保定,071000 |
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| 基金项目： | 河北省教育厅基金资助项目(2003204) |
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| 摘 要： | 采用大肠杆菌与枯草杆菌的穿梭质粒pMK4作为载体,构建了含有果聚糖酶基因(sacB)启动子和vgb基因的重组质粒pMK-SV.通过原生质体和电转化,将pMK-SV转入苏云金杆菌中,经影印筛选得到转化子Bt4.对Bt4诱导表达,与原菌株相比Bt4的生长量增加了20.2%.
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| 关 键 词： | 苏云金杆菌 vgb基因 表达 原生质 电转化 |
| 文章编号： | 1000-1565(2006)01-0033-05 |
| 修稿时间： | 2005-04-10 |
Expression of Vitreoscilla Hemoglobin gene in Bacillus thuringiensis |
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| ZHOU Yan-fen,ZHAO Xiao-yu,ZHANG Yu,FENG Shu-liang,WANG Rong-yan. Expression of Vitreoscilla Hemoglobin gene in Bacillus thuringiensis[J]. Journal of Hebei University (Natural Science Edition), 2006, 26(1): 33-37 |
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| Authors: | ZHOU Yan-fen ZHAO Xiao-yu ZHANG Yu FENG Shu-liang WANG Rong-yan |
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| Abstract: | For efficient expression of vgb gene in Bacillus thuringiensis(Bt),pMK-SV was constructed using pMK4,a shuttle vector between Gram negative and positive,in which vgb gene including sacB promoter was inserted.pMK-SV is transformated into Bt by the method of protoplasm and electroporation.The recombinants were obtained by blotting method.The density of recombinants increases 20.2% compared with original stain(HBF-1) after sugar inducing. |
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| Keywords: | Bacillus thuringiensis vgb gene expression protoplasm electroporation |
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