| 地衣酚法测定RNA的条件试验 |
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| 引用本文： | 朱哲保,高盛科,潘红春. 地衣酚法测定RNA的条件试验[J]. 四川理工学院学报(自然科学版), 1994, 0(1) |
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| 作者姓名： | 朱哲保 高盛科 潘红春 |
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| 摘 要： | 通过地衣酚试剂浓度与用量试验，确定核酸测定的地衣酚浓度为０．２％，试剂用量为样品用量的三倍（反应体系的盐酸浓度为７．２—８．１ｍａｌ／Ｌ）的条件下加热２０分钟，可得到稳定的光密度值。
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| 关 键 词： | 核糖核酸；地衣酚；分光光度法 |
A TEST FOR CONDITI0NS OF QUANTITATION RNA BY SPECTROPHOTOMETRY WITH ORCINOL |
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| Zhu Zhebao, Gao Shengke, Pan HongChun. A TEST FOR CONDITI0NS OF QUANTITATION RNA BY SPECTROPHOTOMETRY WITH ORCINOL[J]. Journal of Sichuan University of Science & Engineering(Natural Science Editton), 1994, 0(1) |
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| Authors: | Zhu Zhebao Gao Shengke Pan HongChun |
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| Affiliation: | Dept.of Light Industry Engineering |
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| Abstract: | A stable optical density value is established by varying concentration and volume of orcinol solution.That is,orcinol solution concentration is 0.2%,its volume is threefold of example(HCl concentration in total reaction system is 7.2-8.1 mal/L),and in the boiled water for 20 minutes. |
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| Keywords: | ribonucleic acid orcinol spectrophotometry |
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