| SARS-CoV S基因表达及纯化 |
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| 引用本文： | 柴明青,张东华,陆志檬,谢幼华,沈忠明. SARS-CoV S基因表达及纯化[J]. 上海师范大学学报(自然科学版), 2004, 33(3): 72-76 |
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| 作者姓名： | 柴明青 张东华 陆志檬 谢幼华 沈忠明 |
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| 作者单位： | 上海大学,生命科学学院,上海,200436;上海第二医科大学,上海,200025;中国科学院上海生命科学研究院,上海,200031 |
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| 摘 要： | 严重急性呼吸道综合症是由一种新的冠状病毒SARS-CoY引起的．作者通过PCR扩增得到了S蛋白的6个编码片段，并利用表达载体pET28a( )在E．coli BL21中进行了原核表达．通过亲和层析纯化了包含大部分ACE2结合区域的S蛋白片段(S4)．ELISA分析结果表明S4与SARS病人恢复期血清具有良好的反应能力．
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| 关 键 词： | SARS S蛋白 表达纯化 血清检测 |
| 文章编号： | 1000-5137(2004)03-0072-05 |
| 修稿时间： | 2004-04-26 |
Expression and purification S protein of SARS-CoV |
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| CHAI Ming-qing,Zhang Dong-hua,LU Zhi-meng,XIE You-hua,SHEN Zhong-ming. Expression and purification S protein of SARS-CoV[J]. Journal of Shanghai Normal University(Natural Sciences), 2004, 33(3): 72-76 |
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| Authors: | CHAI Ming-qing Zhang Dong-hua LU Zhi-meng XIE You-hua SHEN Zhong-ming |
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| Abstract: | A distinct coronavirus (SARS-CoV) has been identified as the etiological agent of Severe Acute Respiratory Syndrome (SARS). We obtained six coding fragments of the SARS-CoV S gene by PCR and synthesized them in Escherichia cob BL21. The S4 protein which contains a large part of the ACE - 2 binding region was purified by nickel - affinity chromatography. ELISA showed that the S4 protein had a good reactivity with the sear from a SARS patient. |
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| Keywords: | SARS S protein expression and purification detection |
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