| 猪细小病毒血清学检测方法的建立 |
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| 引用本文： | 吴淑芳,付瑞,邢瑞昌,贺争鸣.猪细小病毒血清学检测方法的建立[J].实验动物科学,2007,24(2):56-59. |
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| 作者姓名： | 吴淑芳 付瑞 邢瑞昌 贺争鸣 |
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| 作者单位： | 中国药品生物制品检定所国家实验动物微生物、遗传检测中心,北京,100050 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的建立猪细小病毒血清学检测方法。方法用抗猪细小病毒的猪源性血清,通过IFA和IEA两种方法,首先将阳性血清吸附PK-15细胞抗原,以排除非特异反应,通过对阳性血清及3种二抗(其中IFA以FITC标记的抗猪IgG抗体,IEA分别以HRP标记的抗猪IgG抗体和SPA-HRP作为二抗)进行浓度梯度滴定,确定最佳工作浓度,并比较三种反应体系的敏感性。结果IFA最高能检测出PPV-猪组织混合感染PK-15细胞模型中1个TCID50,而其它两种反应体系均只能检测100个TCID50。结论间接免疫荧光检测方法比其它两种方法敏感性更高。
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| 关 键 词： | 猪细小病毒 间接免疫荧光 免疫酶 |
| 文章编号： | 1006-6179（2007）02-0056-04 |
| 收稿时间： | 2006-05-30 |
| 修稿时间： | 2006-05-30 |
Establishment and Application of Serological Test for Porcine Parvovirus |
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| WU Shu-fang,FU Rui,XING Rui-chang,HE Zheng-ming.Establishment and Application of Serological Test for Porcine Parvovirus[J].Shiyan Dongwu Kexue,2007,24(2):56-59. |
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| Authors: | WU Shu-fang FU Rui XING Rui-chang HE Zheng-ming |
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| Institution: | National Institute for the Control of Pharmaceutical and Biological Products, National Center for Microbiological and Genetic Monitoring of Laboratory Animal, Beijing 100050 |
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| Abstract: | ObjectiveTo establish the serological method for detection of porcine parvovirus(PPV).MethodsFirstly,the swine serum containing the antibodies against PPV was absorbed by the PK-15 to eliminate nonspecific reaction,next,the optimal reaction was determined by concentration gradient titration of the serum and the second reaction-antibodies,and then,the sensitivity among the reaction systems was compared.Results 1 TCID_50 could be acquired with IFA,however,only 100 TCID_50 could be acquired with the other two methods. ConclusionIFA method was more sensitive than the other two methods. |
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| Keywords: | Porcine parvovirus IFA IEA |
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