| 不同负荷运动对小鼠骨骼肌HO-1mRNA表达及血清CK、BUN的影响 |
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| 引用本文： | 常凤,陈德明,李彦龙,高旭,李丽,马瑞.不同负荷运动对小鼠骨骼肌HO-1mRNA表达及血清CK、BUN的影响[J].南京体育学院学报(自然科学版),2012,11(1):14-17. |
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| 作者姓名： | 常凤 陈德明 李彦龙 高旭 李丽 马瑞 |
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| 作者单位： | 1. 哈尔滨体育学院教务处,哈尔滨,150008
2. 高旭哈尔滨医科大学生物化学与分子生物学教研室,哈尔滨,150086 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 目的:探讨不同负荷运动对小鼠股四头肌HO-1mRNA表达及血清CK、BUN的影响。方法:饲养、适应性运动BDF1小鼠,6周龄时随机分为对照组、30min、45 min、180min中等强度运动组;45 min低强度、高强度运动组,利用RT-PCR法测定各组HO-1mRNA表达情况,利用动力学法测定血清CK;利用两点法测定血清BUN浓度。结果:(1)中等强度运动可诱导小鼠股四头肌HO-1mRNA表达增加,45min运动组HO-1mRNA诱导最明显;(2)运动诱导HO-1mRNA表达增加与运动时间、运动强度无依赖关系;(3)血清CK、BUN随运动时间、运动强度变化发生改变。结论:运动可以诱导小鼠骨骼肌HO-1mRNA表达增加。
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| 关 键 词： | 运动 小鼠 骨骼肌 HO-1 CK BUN |
Research on the Mice Skeletal Muscle HO-1 Expression Effects in Different Load Exercise and Serum CK、BUN |
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| CHANG Feng,CHEN De-ming,LI Yan-long,GAO Xu,LI Li,MA Rui.Research on the Mice Skeletal Muscle HO-1 Expression Effects in Different Load Exercise and Serum CK、BUN[J].Joournal of Nanjing Institute of Physical Education:Natural Science,2012,11(1):14-17. |
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| Authors: | CHANG Feng CHEN De-ming LI Yan-long GAO Xu LI Li MA Rui |
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| Institution: | 1.Harbin Institute of Physical Education Office of Academic Affairs,Harbin 150008,China;2.Harbin Medical University Department of Biochemistry and Molecular Biology,Harbin150086,China |
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| Abstract: | Objective:Discussion on the mice quadriceps HO-1 expression effects in different load exercise and serum CK、BUN.Method:Feed and adaptive exercise BDF1 mice,They are randomized the control group、30 min、45 min、180 min middle intensity exercise group;45 min low and high intensity exercise group;Measure of the HO-1mRNA expression level of each group by RT-PCR;Measure of Serum CK activity by Kinetic method;Measure of Serum BUN concentration by two-point method.Result:(1)Middle intensity exercise can induce expression of HO-1 mRNA,45min group is the most obvious.(2)It is no dependent relationship that expression of HO-1 mRNA between exercise time and exercise intensity.(3)Serum CK,BUN change with exercise time,exercise intensity.Conclusion:Exercise can induce expression of HO-1 mRNA. |
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| Keywords: | exercise mice skeletal muscle HO-1 CK BUN |
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