| 高效阴离子交换色谱测定蜂蜜中葡萄糖和果糖的研究 |
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| 引用本文： | 胡才龙,李丹,李晓磊. 高效阴离子交换色谱测定蜂蜜中葡萄糖和果糖的研究[J]. 长春大学学报, 2011, 0(10): 56-59 |
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| 作者姓名： | 胡才龙 李丹 李晓磊 |
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| 作者单位： | 吉林农业大学食品科学与工程学院食品工程系;长春大学生物科学技术学院食品工程系; |
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| 摘 要： | 建立离子色谱法检测蜜蜂中糖分的分析方法。用CarboPacTMPAl0（4mm×250mm）阴离子交换分离柱和脉冲安培检测器,对淋洗分离条件进行优化。选择18mmol/LNaOH为淋洗液,淋洗液流速1.0mL/min,总时间20min。该方法能较好地分离葡萄糖、果糖和蔗糖。天然蜂蜜中蔗糖含量非常少,利用此方法可以鉴别天...
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| 关 键 词： | 离子色谱 脉冲安培检测 蜂蜜 |
Research on Determination of Contents of Glucose and Levulose in Honey by High Performance Anion Exchange Chromatography |
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| HU Cai-long,LI Dan,LI Xiao-lei. Research on Determination of Contents of Glucose and Levulose in Honey by High Performance Anion Exchange Chromatography[J]. Journal of Changchun University, 2011, 0(10): 56-59 |
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| Authors: | HU Cai-long LI Dan LI Xiao-lei |
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| Affiliation: | HU Cai-long1,LI Dan2,LI Xiao-lei2(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China,2.Bio-Scientific and Technical College,Changchun University,Changchun 130022,China) |
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| Abstract: | This paper establishes an analysis method of determining sugar content in honey by ion chromatography,which uses CarboPacTMPAl0（4mm×250mm） anion exchange column and pulsed amperometric detector to optimize leaching separation conditions by taking 18mmol/L NaOH as eluate at a flow rate of 1.0 mL/min for 20 min.This method can better separate glucose,fructose and sucrose.The content of sucrose in natural honey is very little.We can identify the authenticity of natural honey by this method. |
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| Keywords: | ion chromatography pulsed amperometric detection honey |
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