Study on flow characteristics of solid/liquid system in lysozyme crystal growth

During the process of lysozyme protein crystallization with batch method,the macroscopic flow field of solid/liquid system was observed by particle image velocimetry(PIV). Furthermore,a normal growth rate of(110) face and local flow field around a single protein crystal were obtained by a long work dis-tance microscope. The experimental results showed that the average velocity,the maximal velocity of macroscopic solid/liquid system and the velocity of local flow field around single protein crystal were fluctuant. The effective boundary layer thickness δeff,the concentration at the interface Ci and the characteristic velocity V were calculated using a convection-diffusion model. The results showed that the growth of lysozyme crystal in this experiment was dominated by interfacial kinetics rather than bulk transport,and the function of buoyancy-driven flow in bulk transport was small,however,the effect of bulk transport in crystal growth had a tendency to increase with the increase of lysozyme concentra-tion. The calculated results also showed that the order of magnitude of shear force was about 10-21 N,which was much less than the bond force between the lysozyme molecules. Therefore the shear force induced by buoyancy-driven flows cannot remove the protein molecules from the interface of crystal.

Study on flow characteristics of solid/liquid system in lysozyme crystal growth

During the process of lysozyme protein crystallization with batch method, the macroscopic flow field of solid/liquid system was observed by particle image velocimetry (PIV). Furthermore, a normal growth rate of (110) face and local flow field around a single protein crystal were obtained by a long work distance microscope. The experimental results showed that the average velocity, the maximal velocity of macroscopic solid/liquid system and the velocity of local flow field around single protein crystal were fluctuant. The effective boundary layer thickness δ _eff, the concentration at the interface C _i and the characteristic velocity V were calculated using a convection-diffusion model. The results showed that the growth of lysozyme crystal in this experiment was dominated by interfacial kinetics rather than bulk transport, and the function of buoyancy-driven flow in bulk transport was small, however, the effect of bulk transport in crystal growth had a tendency to increase with the increase of lysozyme concentration. The calculated results also showed that the order of magnitude of shear force was about 10⁻²¹ N, which was much less than the bond force between the lysozyme molecules. Therefore the shear force induced by buoyancy-driven flows cannot remove the protein molecules from the interface of crystal. Supported by the National Natural Science Foundation of China (Grant Nos. 10472127 and 10432060) and the Knowledge Innovation Program of Chinese Academy of Sciences (Grant Nos. KSCX2-SW-322 and KJCX2-SW-L05)