| 外源基因在球形芽孢杆菌中的转化 |
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| 引用本文： | 张培德,苑小林.外源基因在球形芽孢杆菌中的转化[J].复旦学报(自然科学版),1995,34(4):417-422. |
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| 作者姓名： | 张培德 苑小林 |
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| 作者单位： | 复旦大学生物化学系 |
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| 摘 要： | 为了扩大芽孢杆菌属的受体系统范围，对球形芽孢杆菌进行了质粒消除，并用含有溶葡球菌酶基因的质粒ｐＥ１９４ｃｏｐ６－１转化，结果表明，该酶基因在球形芽孢杆菌中获得了稳定，高效表达。
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| 关 键 词： | 球形芽孢杆菌 质粒消除 外源基因 蛋白酶 |
Elimination of plasmid and transformation into Bacillus sphaericus |
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| Zhang Peide,Yuan Xiaolin,Chen Shigen.Elimination of plasmid and transformation into Bacillus sphaericus[J].Journal of Fudan University(Natural Science),1995,34(4):417-422. |
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| Authors: | Zhang Peide Yuan Xiaolin Chen Shigen |
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| Institution: | Deptartment of Biochemistry |
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| Abstract: | In order to expend the host system,plasmid in B. sphaericus was eliminated and then was transformed with plasmid pE194 cop 6-1 containing lysostaphin gene into this strain. It was showed that the lysostaphin gene could express stably in high level in Bacillus sphaericus. |
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| Keywords: | Bacillus sphaericus eliminating plasmid |
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