| 福寿螺糖苷水解酶基因核心片断的克隆及序列分析 |
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| 引用本文： | 欧阳嘉,谢承佳,李双,何冰芳.福寿螺糖苷水解酶基因核心片断的克隆及序列分析[J].南京林业大学学报(自然科学版),2006,30(2):29-32. |
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| 作者姓名： | 欧阳嘉 谢承佳 李双 何冰芳 |
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| 作者单位： | 1. 南京林业大学化学工程学院,江苏,南京,210037;南京工业大学制药与生命科学学院,江苏,南京,210009
2. 南京工业大学制药与生命科学学院,江苏,南京,210009 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家高技术研究发展计划(863计划) |
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| 摘 要： | 从福寿螺的胃部组织中获得总RNA,经RT-PCR扩增、测序得到1条新糖苷水解酶基因片段。分析显示其ORF(Open Reading Frame)全长882 bp,编码294个氨基酸。与福寿螺多功能纤维素酶EGX的C端高度同源性达到89.8%,含有糖苷水解酶第10家族的保守性氨基酸序列。
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| 关 键 词： | 福寿螺 糖苷水解酶 纤维素 木聚糖 |
| 文章编号： | 1000-2006(2006)02-0029-04 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005-12-15 |
Cloning and Sequencing of a Molluscan Glycosyl Hydrolase Core Gene from the Snail,Ampullaria crossean |
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| OUYANG Jia,XIE Cheng-jia,LI Shuang,HE Bing-fang.Cloning and Sequencing of a Molluscan Glycosyl Hydrolase Core Gene from the Snail,Ampullaria crossean[J].Journal of Nanjing Forestry University(Natural Sciences ),2006,30(2):29-32. |
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| Authors: | OUYANG Jia XIE Cheng-jia LI Shuang HE Bing-fang |
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| Institution: | 1. College of Chemical Engineering Nanjing Forestry University, Nanjing 210037 ,China; 2. College of Life Science and Pharmacy Nanjing Technology University , Nanjing 210009, China |
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| Abstract: | As a mollusk degrading the plant biomass rapidly,Ampullaria crossean can produce cellulose-hydrolytic enzymes.This brings us a new sight into the biological degradation of the plant biomass.Total RNA was isolated from the stomach of the mollusk Ampullaria crossean by using polymerase chain reaction,cloning and sequencing techniques,a complementary DNA DY1300 encoding a low molecular mass glycosyl hydrolase has been identified.It contains a 882-nucleotide ORF encoding a 294 amino-acid protein.The percentage of similarity with the cellulase EGX gene sequence from Ampullaria crossean is 89.8%.Amino acid sequence-based classification has revealed that the putative protein belongs to the glycosyl hydrolase family 10(GHF10). |
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| Keywords: | Glycosyl hydrolase Ampullaria crossean Cellulose Xylan |
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