不同氮磷浓度对铜绿微囊藻生长特性的影响

在不同氮（N）、磷（P）初始浓度的培养液中对铜绿微囊藻（Microcystis aeruginosa）进行培养.利用Monod方程分别计算铜绿微囊藻对氮、磷的半饱和常数（Ks）.结果表明：以单一元素为限制底物时，满足铜绿微囊藻正常生长的氮浓度大于4.0 mg·L^-1，磷浓度大于0.50 mg·L^-1；铜绿微囊藻最适生长的氮浓度范围为32.0~64.0 mg·L^-1，磷浓度范围为1.0~1.50 mg·L^-1；以磷为限制底物时的半饱和常数Ks_p远远小于以氮为限制底物时的半饱和常数Ks_N(Ks_N>Ks_p)，说明铜绿微囊藻对磷的亲和性高于氮.与铜绿微囊藻最大现存量（X）呈高度线性相关时的氮浓度范围为0.20~64.0 mg·L^-1，磷浓度范围为0.02~1.50 mg·L^-1.铜绿微囊藻比增长速率（μ）连续增加的氮浓度范围为0.20~1.60 mg·L^-1，磷浓度范围为0.02~0.50 mg·L^-1.

Influence of nitrogen and phosphorus concentrations on the growth characteristics of Microcystis aeruginosa

Microystis aeruginosa was cultured in solutions of different initial nitrogen and phosphorus concentrations.The semi-saturation constants（Ks） of Microystis aeruginosa to P and N were calculated respectively by using the Monod equation.The results show that the Microystis aeruginosa grew normally when the concentrations of N and P were greater than 4.0 mg·L^-1 and 0.50 mg·L^-1 respectively.The optimum concentration ranges of N and P for Microystis aeruginosa growth were 32.0～64.0 mg·L^-1 and 1.0～1.50 mg·L^-1 respectively.Ks_N is higher than K_sp,which indicates that the affinity of P to Microystis aeruginosa is higher than N. When the concentration ranges of N and P were 0.20～64.0 mg·L^-1 and 0.02～1.50 mg·L^-1 respectively,they had high liner correlation with the largest extent quantity of Microystis aeruginosa. The special growth rate increases continuously when the concentration ranges of N and P are 0.20～Y1.60 mg·L^-1 and 0.02～0.50 mg·L^-1 respectively.