Monoclonal antibodies to L-asparaginase |
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Authors: | T. Kitao K. Hattori |
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Affiliation: | (1) Department of Medicine, Kanazawa University School of Medicine, 920 Kanazawa, (Japan) |
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Abstract: | Summary Five hybridomas secreting monoclonal antibody toE. coli L-asparaginase were isolated. These monoclonal antibodies were classified into 3 different subclasses; Ig G1 (1 clone), Ig G2 (2 clones) and Ig G3 (2 clones). One of them possessed anti-L-asparaginase neutralizing activity. Four antibodies examined demonstrated a linear Langmuir binding plot and binding affinities, with equilibrium dissociation constant (Kd) ranging between 2.5×10–9M and 6.3×10–10 M. The monoclonal antibodies should be useful probes for investigation of the enzyme activity. |
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