微氧条件下固定化颗粒污泥的氯酚降解及菌群结构

将氯酚优势好氧菌与厌氧颗粒污泥以三种不同方式组合：（1）以聚乙烯醇和海藻酸钠为载体，采用冷冻法将氯酚优势好氧菌和厌氧颗粒污泥进行混合固定；（2）将氯酚优势好氧菌单独固定后再与厌氧颗粒污泥混合；（3）将氯酚优势好氧菌直接投加到厌氧颗粒污泥．比较三种固定化颗粒污泥在微好氧及厌氧条件下的五氯酚降解效果．结果表明：在氯酚优势好氧菌和厌氧颗粒污泥的混合固定中，微好氧条件下可通过厌氧菌与好氧菌的协同作用实现氯酚的厌氧好氧同时降解，而在厌氧过程中，三种固定化颗粒污泥的氯酚降解速率没有明显差别．厌氧好氧活性实验也进一步证实了固定化颗粒污泥中厌氧、好氧菌活性的存在，并进一步推导出了微氧条件下氯酚固定化颗粒污泥的菌群结构．

Chlorophenol Degradation and Microorganism Community Structure of Immobilized Granular Sludge Under Micro-Aeration Condition

A comparison of PCP(Pentachlorophenol) degradation was made in micro-aeration and anaerobic conditions with three series of batch experiments:(1) anaerobic granular sludge and the mixed isolated aerobic bacteria were co-immobilized with PVA(Polyvinyl Alcohol) and sodium alginate by means of freeze;(2) isolated aerobic bacteria were first immobilized with PVA and sodium alginate and then mixed with anaerobic granular sludge;and(3) isolated aerobic bacteria were directly added in anaerobic granular sludge.The results indicate that,in the micro-aeration condition,the co-immobilization of anaerobic granular sludge and isolated aerobic bacteria can enhance the PCP degradation through the synergism of aerobes and anaerobes.Whereas in the anaerobic condition,there is no significant difference in degradation rate among the three immobilized anaerobic granular sludge.Moreover,the presence of the activities of aerobes and anaerobes in the immobilized sludge is further conformed by specific activity experiments in aerobic and anaerobic conditions,and the microorganism community structure of the co-immobilized granular sludge in micro-aeration condition is also deduced.