高糖饮食及高脂饮食建立非酒精性脂肪肝大鼠模型的比较

目的探讨高脂饮食及高糖饮食在建立非酒精性脂肪肝大鼠模型上的异同。方法分别给予雄性SD大鼠正常饮食、高糖饮食、高脂饮食,于48、、12周处死,光镜下观察肝脏病理变化,检测肝重指数、ALT、AST、CHO、TG、稳态模型评估胰岛素抵抗(HOMA-IR)指标。结果高糖饮食及高脂饮食大鼠的肝脏均出现脂肪浸润,并且随着时间的延长程度逐渐加重,以高脂组为明显。高脂组在12周时肝脏出现炎性细胞浸润,并且转氨酶升高,说明进入非酒精性脂肪性肝炎(NASH)阶段。高脂组血脂以CHO升高为主,高糖组血脂以TG升高为主。高脂组在4、8周HOMA-IR均高于高糖组,与病理变化相平行,12周时低于高糖组,提示尚有其他因素参与单纯性脂肪肝向肝炎的转变。结论高糖饮食及高脂饮食均能成功诱导大鼠出现非酒精性脂肪肝,在肝脏病理、各生化指标及HOMA-IR指数上有各自的特点。

The Difference between the Rat Nonalcoholic Fatty Liver Models Induced by High-fat Diet and High-sucrose Diet

Objective To study the difference between the rat nonalcoholic fatty liver models induced by high-fat diet and high-sucrose diet.Methods 90 SD rats were randomly divided into three groups of 30 rats each and were fed with nomal diet,high-fat diet and high-sucrose diet respectively.10 rats in each group were sacrificed at 4~(th),8~(th) and 12~(th) week.The level of ALT,AST,CHO,TG and HOMA-IR were investigated and the pathology of livers were observed by HE stain.Result The liver in both high-fat diet and high-sucrose diet group presented the pathology of steatosis and got more severe along with the time,especially the high-fat diet group.The rats' livers in high-fat group presented the pathology of steatohepatitis with higher serum levels of ALT and AST in the 12~(th) week.The CHO level in high-fat diet group and the TG level in high-sucrose group were significantly higher than control group.The HOMA-IR in high-fat group was significantly higher than high-sucrose group at 4~(th) and 8~(th) week,peraleled with the liver pathology.But at the 12~(th) week the HOMA-IR in high-fat group was lower than high-sucrose group significantly suggesting that other facters may also involve in the progression from simple steatosis to steatohepatitis.Conclusions High-fat and high-sucrose diet can induce nonalcoholic fatty liver rat model successfully and have different characteristics each.