| IL－10与放线菌酮诱导K562细胞凋亡 |
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| 引用本文： | 狄静芳,曾山,冯铮,曾耀英,曹艳英.IL－10与放线菌酮诱导K562细胞凋亡[J].暨南大学学报,2001,22(4):10-13. |
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| 作者姓名： | 狄静芳 曾山 冯铮 曾耀英 曹艳英 |
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| 作者单位： | 暨南大学组织移植免疫中心, |
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| 摘 要： | 目的：研究IL－10对放线菌酮（CHX）诱导K562细菌凋亡所产生的调节作用。方法：采用碘化丙啶（PI）染色流式细胞仪分析，形态学观察及DNA凝胶电泳检测K562细胞。结果：CHX组K562凋亡细胞达58．3％，CHX加IL－10组达75．8％。结论：（1）CHX能诱导K562细胞凋亡。（2）IL－10和CHX联合作用诱导K562细胞凋亡的作用增强。
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| 关 键 词： | 白细胞介素10 放线菌酮 细胞凋亡 形态学 流式细胞仪 肿瘤治疗 红白血病 |
| 文章编号： | 1000-9965(2001)04-0010-04 |
The research on IL-10 and cycloheximide inducing K562 cell apoptosis |
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| DI Jing-fang,ZHENG Shan,FENG Zeng,ZHENG Yao-ying,CAO Yan-ying.The research on IL-10 and cycloheximide inducing K562 cell apoptosis[J].Journal of Jinan University(Natural Science & Medicine Edition),2001,22(4):10-13. |
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| Authors: | DI Jing-fang ZHENG Shan FENG Zeng ZHENG Yao-ying CAO Yan-ying |
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| Abstract: | Aim: To evaluate the effects of IL-10 and cycloheximide(CHX) inducing K562 cell apoptosis. WTHZMethods:WTBZ K562 cell was examined using FCM analysis?Light microscopy with staining Hoechst 33342 and DNA ladder methods. WTHZResults:WTBZ K562 apoptotic cell in CHX group was 58.3%. But apoptotic cell in CHX and IL-10 group reached 75.8%. WTHZConclusions:WTBZ(1) CHX could induce the apoptosis of K562 cell strongly. (2) Combined use of IL-10 and CHX had the synergetic effect in inducing the apoptosis of K562.(3) IL-10 had no evident effect in inducing the apoptosis of K562. |
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| Keywords: | IL-10 cycloheximide cell apoptosis morphology flow cytometry |
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