Mapping the Nuclear Fertility Restorer Gene in Rice with Simple Sequence Length Polymorphism (SSLP) |
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引用本文: | 朱建清,Zhang Hanyao,Liu Zhu,Liao Jinhua,LI Shujin,Ye Xiaoying,Wang Lihua,Zhao Jian,Yang Zhirong. Mapping the Nuclear Fertility Restorer Gene in Rice with Simple Sequence Length Polymorphism (SSLP)[J]. 高技术通讯(英文版), 2004, 10(4): 94-96 |
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作者姓名: | 朱建清 Zhang Hanyao Liu Zhu Liao Jinhua LI Shujin Ye Xiaoying Wang Lihua Zhao Jian Yang Zhirong |
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作者单位: | [1]LifeSciencecollege,S'ichuanUniversity,Chengdu610064,P.R.China [2]RiceResearchInstitute,SichuanAgriculturalUniversity,Wenjiang611130,P.R.China [3]LifeSciencecollege,SichuanUniversity,Chengdu610064,P.R.Chin//RiceResearchInstitute,SichuanAgriculturalUniversity,Wenjiang611130,P.R.China |
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基金项目: | SupportedbytheHighTechnologyResearchandDevelopmentProgrammeofChina |
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摘 要: | Bulked segregant analysis (BSA) of a F2 population derived from D62A/Ruby B was used to map the nuclear fertility restorer gene for wild abortive (WA) cytoplasmic male sterility. Three hundred and ninety-seven microsatellite primer pairs which distributed on 12 chromosomes were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM182 located on chromosome 7 produced polymorphic products. The nuclear fertility restorer gene for WA cytoplasmic male sterility was mapped on chromosome 7, 7.4cM from RM182.
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关 键 词: | 稻 细胞质 雄性不育基因 修复因子 多态现象 |
Mapping the Nuclear Fertility Restorer Gene in Rice with Simple Sequence Length Polymorphism (SSLP) |
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Affiliation: | Life Science college, Sichuan University, Chengdu 610064, P.R.China;Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, P.R.China;Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, P.R.China;Life Science college, Sichuan University, Chengdu 610064, P.R.China |
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Abstract: | Bulked segregant analysis (BSA) of a F_2 population derived from D62A/Ruby B was used to map the nuclear fertility restorer gene for wild abortive (WA) cytoplasmic male sterility. Three hundred and ninety-seven microsatellite primer pairs which distributed on 12 chromosomes were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM182 located on chromosome 7 produced polymorphic products. The nuclear fertility restorer gene for WA cytoplasmic male sterility was mapped on chromosome 7, 7.4cM from RM182. |
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Keywords: | rice cytoplasmic male sterility restorer gene Simple Sequence Length Polymorphism |
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