| 盐生杜氏藻Ugd基因的cDNA克隆及序列分析 |
| |
| 引用本文： | 李音,乔代蓉,易弋,贺庆华,李良,孙辉,曹毅.盐生杜氏藻Ugd基因的cDNA克隆及序列分析[J].四川大学学报(自然科学版),2004,41(2):409-412. |
| |
| 作者姓名： | 李音 乔代蓉 易弋 贺庆华 李良 孙辉 曹毅 |
| |
| 作者单位： | 四川大学生命科学学院,成都,610064 |
| |
| 基金项目： | 国家863项目(2002AA213021)，国家自然科学基金(30270711) |
| |
| 摘 要： | 作者对不同物种Ugd基因的同源序列进行相似性分析后设计一对兼并引物，利用RTPCR技术获得一条200bp左右的片段，测序分析显示其同芋头(Colocasia esculenta)的Ugd基因的编码区有71．7％的相似性．然后再以此片段为模板设计引物，通过RACE技术获得盐生杜氏藻Ugd基因的全长序列．经克隆测序作blastx分析发现其同芋头(Colocasia esculenta)、大豆(Soybean)、水稻(Oryza sativa)、拟南芥(Arabidopsis thaliana)的Ugd基因有78％到81％的同源性．
|
| 关 键 词： | 同源克隆 兼并引物 Ugd RACE |
| 文章编号： | 0490-6756(2004)02-0409-04 |
Molecular cloning and sequence analysis of Ugd gene in Dunaliella.Salina |
| |
| LI Yin,QIAO Dai-rong,YI Yi,HE Qing-hua,Li Liang,SUN Hui,CAO Yi.Molecular cloning and sequence analysis of Ugd gene in Dunaliella.Salina[J].Journal of Sichuan University (Natural Science Edition),2004,41(2):409-412. |
| |
| Authors: | LI Yin QIAO Dai-rong YI Yi HE Qing-hua Li Liang SUN Hui CAO Yi |
| |
| Abstract: | The degenerate primers were designed based on the homologous gene of Ugd in other organisms.A cDNA fragment of 200bp from D.salina was obtained by RT-PCR technique. After cloning and DNA sequencing, the result indicated that 71.7% of the sequence of the fragment was homologous to the Ugd in Colocasia esculenta.Based on this sequence, a pairs of primers weredesigned. By the RACE technique, the full-length cDNA of Ugd was successfully constructed in D.salina. |
| |
| Keywords: | homologous cloning degenerate primer Ugd(UDP-glucose dehydrogenase) RACE(Rapid Amplification of cDNA End) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
