| 产甘油重组大肠杆菌的构建及其耐高渗透压性能的测定 |
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| 引用本文： | 萨娜,王熙,赵鹏,葛喜珍,田平芳.产甘油重组大肠杆菌的构建及其耐高渗透压性能的测定[J].北京化工大学学报(自然科学版),2011,38(6):93-97. |
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| 作者姓名： | 萨娜 王熙 赵鹏 葛喜珍 田平芳 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京联合大学生物化学工程学院,北京,100023 |
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| 基金项目： | 国家自然科学基金(20876009/21076013) |
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| 摘 要： | 克隆了酿酒酵母(Saccharomyces cerevisiae) 中的3-磷酸甘油脱氢酶和3-磷酸甘油酯酶基因，通过融合PCR构建了双基因共表达载体，将酵母细胞内应答渗透压变化的甘油合成途径引入大肠杆菌（Escherichia coli）。以葡萄糖为底物对重组大肠杆菌进行摇瓶发酵培养，该重组菌的甘油产量为1g/L。渗透压胁迫测试证明该重组菌的耐渗透压性能较出发菌株有明显提高。
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| 关 键 词： | 大肠杆菌 甘油 渗透压 3-磷酸甘油脱氢酶 3-磷酸甘油酯酶 |
| 收稿时间： | 2011-05-27 |
Engineering Escherichia coli for production of glycerol and assay for its tolerance to high osmosis |
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| SA Na,WANG Xi,ZHAO Peng,GE XiZhen,TIAN PingFang.Engineering Escherichia coli for production of glycerol and assay for its tolerance to high osmosis[J].Journal of Beijing University of Chemical Technology,2011,38(6):93-97. |
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| Authors: | SA Na WANG Xi ZHAO Peng GE XiZhen TIAN PingFang |
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| Institution: | 1.College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029;2.Biochemical Engineering College, Beijing Union University, Beijing 100023, China |
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| Abstract: | A pathway for glycerol synthesis has been introduced into Escherichia coli BL-21 by constructing a co-expression vector harbouring genes encoding glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase that govern the glycerol production in Saccharomyces cerevisiae.As a result,a glycerol concentration of 1g/L was achieved in flask culture and the recombinant species could regulate intracellular osmotic pressure and resist environmental stress in the synthesis of glycerol in vivo. |
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| Keywords: | Escherichia coli glycerol osmosis glycerol-3-phosphate dehydrogenase glycerol-3-phosphatase |
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