| 用扫描电镜观察裸甲藻的方法研究 |
| |
| 引用本文： | 赖红艳,侯建军,王振河.用扫描电镜观察裸甲藻的方法研究[J].湖北民族学院学报(哲学社会科学版),2007,25(3):338-342. |
| |
| 作者姓名： | 赖红艳 侯建军 王振河 |
| |
| 作者单位： | 华中农业大学水产学院，华中农业大学水产学院，河南科技学院生物工程系 湖北武汉430070，湖北民族学院医学院，湖北恩施445000，湖北武汉430070，湖北民族学院医学院，湖北恩施445000，河南新乡453003 |
| |
| 基金项目： | 国家自然科学基金
,
中国博士后科学基金 |
| |
| 摘 要： | 将多聚甲醛固定的裸甲藻(Takayama pulchellum,TPXM)细胞采用离心法收集,进行小梯度的酒精结合离心法逐级脱水,临界点干燥并真空喷金后,能够在扫描电镜(SEM)下很好地显示出T.pulchellum细胞的完整形态和细微结构,如纵鞭毛、横沟、顶沟、顶突、带状物、藻体线纹、腰鞭毛等;同时环境扫描电镜(ESEM)也能对裸甲藻进行较好的形态学观察,但环境样品的ESEM和SEM电镜观察条件尚需要进一步完善.
|
| 关 键 词： | 扫描电镜 环境扫描电镜 裸甲藻 细胞形态学 方法 |
| 文章编号： | 1008-8423（2007）03-0338-05 |
| 收稿时间： | 2007-04-02 |
| 修稿时间： | 2007年4月2日 |
Research on the Method of Observing Takayama pulchellum with Scanning Electron Microscopic |
| |
| LAI Hong-yan,HOU Jian-jun,WANG Zhen-he.Research on the Method of Observing Takayama pulchellum with Scanning Electron Microscopic[J].Journal of Hubei Institute for Nationalities(Natural Sciences),2007,25(3):338-342. |
| |
| Authors: | LAI Hong-yan HOU Jian-jun WANG Zhen-he |
| |
| Institution: | 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 2. Collgege of Medicine, Hubei Institute for Nationalities, Enshi 445000, China; 3. Henan Institute of Science and Technology, Xinxiang 453003, China |
| |
| Abstract: | Takayama pulchellum cells fixed by paraformaldehyde(1% final concentration) were collected by centrifugal methods,dehydrated by small grads ethanol concentration and centrifugated again,then evaporation under critical point,spray gold in vacuum.The integrated and detailed structure characteristics of T.pulchellum cells were shown clearly under scanning electron microscope,such as longitudinal flagella,transverse groove,apical groove,apical carina,cingulum,spiral marking,transverse flagella etc.In the meantime,environmental scanning electron microscope was a good tool to observe the morphological characteristics of T.pulchellum cells,but the protocols of ESEM and SEM used to investigate natural samples still need to be optimized. |
| |
| Keywords: | scanning electron microscope environmental scanning electron microscope Takayama pulchellum(TPXM) morphocytology methods |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
