聚丙烯酰胺凝胶电泳——曙红Y染色法

十二烷基硫酸钠—聚丙烯酰胺凝胶电泳已广泛用于分子生物学和医学临床等领域,但该技术的染色方法多沿用考马斯亮蓝和银染色,这两种方法均有其局限性,如蛋白凝胶染色前须经酸或醛固定,不易洗脱且操作繁琐,脱色耗时等。而曙红Y染色具有检测灵敏度高,操作简便,染色时间短,可对各种蛋白质染色,染色后的蛋白质可直接进行电泳转移印迹,也可将凝胶中蛋白质电泳转移后染色。染色后的蛋白质仍具有抗原性。此外,还具有试剂便宜,使用安全,便于推广应用等优点。

Polyacnylamide gel electrophoresis-Eosin Y staining

Sodium dodecyl sulfate-polyacrylamide gel dectrophoresis has been widely applied to the fields of molecular biology and medical clinic. The stainings in that technique, however, as a tradition were mostly Coomassie blue stain and Silver stain, which both show limitations, e. g. , protein gel before staining should be fixed with an acid or aldehyde, it is uneasy to washout with complicated manipulation and time wasting decoloration, etc. , whereas Eosin Y staining exhibits the advantages as high detection sensitivity, simple manipulation, short staining time, being able to stain various kinds of proteins, being able to apply Western blot directly for the protein after staining or to stain the protein in gel after electrophoretic transfer, and the protein after staining still exhibiting antigenicity. In addition, it also exhibits the advantages of cheap reagents,safe application, convenient popularization, etc.