内毒素诱导急性肺损伤小鼠支气管肺泡灌洗液中谷氨酸含量变化的初步研究

目的 观察LPS诱导急性肺损伤时肺内是否存在谷氨酸（Glu）的释放.方法 腹腔注射内毒素制备小鼠急性肺损伤模型,于6h后采用生理盐水行支气管肺泡灌洗,用高效液相色谱法测量灌洗液中氨基酸的浓度.结果 与生理盐水对照组相比,LPS处理组支气管肺泡灌洗液中Glu的浓度增高（P〈0.01）;苏氨酸、蛋氨酸及精氨酸的浓度降低（P〈0.05）,其它氨基酸浓度无显著性变化.结论 在LPS诱导的肺损伤过程中肺组织中部分氨基酸代谢发生显著变化,肺内谷氨酸的释放增多,进一步提示Glu参与了内毒素诱导的肺损伤的病理生理过程.

Change of glutamate content in bronchoalveolar lavage fluid from mice with acute lung injury induced by LPS

Objective To observe the possible release of glutamate in lungs on the acute lung injury induced by lipopolysaccharide（LPS）. Methods The mice models of acute lung injury were created by injecting LPS into abdominal cavity, the bronchoalveolar lavage fluid （BALF）was prepared with nonnal saline 6 hours after the administration of the LPS, the concentration of amino acids in the BALF was measured with high - performance liquid chromatography（ HPLC ）. Results The treatment of LPS injection could effectively increase the concentration of glutamate in BALF（ P 〈 0.01）, decrease the concentration of threonine, methionine and arginine in BALF （ P 〈 0.05 ）, the concentration of other amino acids have no significantly change. Conclusion The metabolism of some amino acids in lungs has been significantly changed on the acute lung injury caused by LPS, the increase of glutmate releasing from lungs further points glutamate involves in the pathophysiological process of LPS - induced lung injury.