Sulfhydryl oxidation induces calcium release from fragmented sarcoplasmic reticulum even in the presence of glutathione |
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Authors: | M Koshita K Miwa T Oba |
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Institution: | (1) Department of Physiology, Nagoya City University Medical School, Mizuho-ku, 467 Nagoya, (Japan) |
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Abstract: | Alcian blue and plumbagin induced transient Ca2+ release from fragmented sarcoplasmic reticulum. Dithiothreitol (DTT) and glutathione (GSH) partially blocked Ca2+ release induced by these oxidizing compounds. Pretreatment of alcian blue and plumbagin with DTT or GSH for more than 1 min was required to abolish the ability of the oxidizing compounds to release Ca2+. Mg2+ and ruthenium red completely blocked alcian blue-and plumbagin-induced Ca2+ release. These results suggest that oxidation of sulfhydryls on Ca2+ release channels induces Ca2+ release even in the presence of GSH in situ. |
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Keywords: | Sarcoplasmic reticulum Ca2+ release sulfhydryl oxidation alcian blue plumbagin |
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