基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测

半胱氨酸（Cys）在生物体系中起着不可缺少的作用，可以参与人体内的许多生理和病理过程，对氧化还原稳态和细胞活性的调节有重要作用。大量研究表明，Cys浓度异常与许多疾病的发生密不可分，因此有必要开发特异性检测Cys的荧光探针。本文以丙烯酰基为识别单元，以二氰基异佛尔酮为荧光基团，设计合成了一种荧光探针ISO-EV-AC。实验结果表明，探针ISO-EV-AC对Cys的检测展现出较高的选择性，不受其他分析物的干扰，检测限低至31 nmol/L，响应Cys的时间为5 min。细胞实验结果表明探针ISO-EV-AC可用于检测细胞中的Cys。

A Fluorescent Probe Based on Isophorone Dicyanide for the Detection of Cysteine in Cells

Cysteine (Cys) plays an indispensable role in biological systems, which can participate in a variety of physiological and pathological processes in the human body and plays an essential role in the regulation of redox homeostasis and cell activity. A large number of studies have shown that abnormal Cys concentrations are strongly associated with the occurrence of various diseases. Therefore, it is necessary to develop a specific fluorescence probe for Cys detection. In this paper, a fluorescent probe ISO-EV-AC was designed and synthesized using acryloyl group as the recognition unit and isophorone dicyano as the fluorophore. The results indicated that probe ISO-EV-AC exhibits a high selectivity for the detection of Cys without interference from the other nanlytes. The detection limit was as low as 31 nmol/L and the response time of Cys was 5 min. The results of the cell experiments demonstrated that probe ISO-EV-AC can be used to detect Cys in cells.