含有Ag颗粒的高灵敏度葡萄糖氧化酶电极的研制

研制了含有ｎｍ级Ａｇ颗粒的葡萄糖氧化酶电极。结果表明，该电极具有很大的响应电流，在相同的条件下，其响应电流比文献所报导的含有Ａｕ或ＳｉＯ２的酶电极响应流大。

A HIGHLY SENSITIVE ENZYME ELECTRODE BY GLUCOSE OXIDASE IMMOBILIZED WITHIN Ag SOL

An amperometric enzyme electrode based on immobilization of glucose oxidase(GOD) within silver(Ag) nanoparticles is presented. The enzyme electrodes are platinum electrodes covered with a membrane containing GOD and Ag nanoparticles. The results show that this kind of enzyme electrodes possess higher sensitivity than those containing Au or SiO 2 nanoparticles. Comparing the response current of these Ag GOD electrodes, it is found that the sensitivity of the enzyme electrodes increases with increasing Ag size in the case of 4.3 7.8 nm. In the case of Ag size increasing continously, the situation is different, the enzyme electrodes have lower sensitivity with increasing Ag size. The mechanism of this phenomenon is studied and discussed on the basis of the ability of Ag nanoparticles transfer electron and the adsorption of GOD onto ultrafine particles.