| 弱精症患者精子miRNA表达谱的构建和分析 |
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| 引用本文： | 陈德宇,刘利敏,黄天华.弱精症患者精子miRNA表达谱的构建和分析[J].阜阳师范学院学报(自然科学版),2009,26(2):47-51. |
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| 作者姓名： | 陈德宇 刘利敏 黄天华 |
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| 作者单位： | 1. 汕头大学医学院,生殖医学研究中心,广东,汕头,515041;阜阳师范学院,安徽,阜阳,236041
2. 汕头大学医学院,生殖医学研究中心,广东,汕头,515041 |
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| 基金项目： | 广东省自然科学基金，广东省科技计划项目 |
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| 摘 要： | 应用基因芯片技术构建弱精症患者精子中miRNA表达谱．收集30份弱精症患者精液标本和20份成年健康有生育能力男性的精液标本，提取精子RNA，标记后，与miRCURY^TM Array芯片杂交．分析弱精症患者精于中miRNA表达情况．高活力精子和低活力精子共196个miRNA存在表达差异，上调miRNA93个，下调miRNA103个．弱精症患者精子miRNA表达谱的建立对分析精子运动的分子机制和探讨弱精症的病因将会有所帮助．
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| 关 键 词： | 弱精症 精子 基因芯片 miRNA |
Construction and analysis of miRNA profiles in the sperm of patients with asthenospermia |
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| CHEN De-yu,LIU Li-min,HUANG Tian-hua.Construction and analysis of miRNA profiles in the sperm of patients with asthenospermia[J].Journal of Fuyang Teachers College:Natural Science,2009,26(2):47-51. |
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| Authors: | CHEN De-yu LIU Li-min HUANG Tian-hua |
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| Institution: | CHEN De-yu1,2,LIU Li-min1,HUANG Tian-hua1 (1.Research Center for Reproductive Medicine,Department of Cell Biology and Genetics,Shantou University Medical College,Shantou Guangdong 515041,China,2.Fuyang Teachers College,Fuyang Anhui 236041,China) |
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| Abstract: | To construct and analyze the miRNA profiles in asthenospermia patients′ sperm.30 semen samples from asthenospermia patients and 20 semen samples from healthy normal adult men were collected,respectively,and then to extract the total RNAs and to produce probes.Hybridization with miRCURYTM Array was carried out.93 miRNAs were up-regulated,103 miRNAs were down-regulated.Construction of miRNA profiles in asthenospermia patients' sperm can help to analyze the molecular mechanism of sperm motility and to study the etiopathogenisis of asthenospermia. |
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| Keywords: | miRNA |
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