| 叶片衰老抑制基因PSAG12-ipt转入籼稻不育系中A的研究 |
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| 引用本文： | 王亚琴,梁承邺.叶片衰老抑制基因PSAG12-ipt转入籼稻不育系中A的研究[J].中山大学学报(自然科学版),2005,44(2):78-81. |
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| 作者姓名： | 王亚琴 梁承邺 |
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| 作者单位： | 1. 华南理工大学,生物科学与工程学院,广东,广州,510640
2. 中国科学院华南植物研究所,广东,广州,510650 |
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| 基金项目： | 广东省重大科技专项基金 |
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| 摘 要： | 以质粒pSG516(ipt)为目的基因, pHQ9(hpt和gus)为选择/标记基因进行共转化,以PSD-1000-氦气基因枪介导,将ipt、hpt和gus基因转化到籼型不育系中A的核DNA中,得到了98个潮霉素抗性再生植株系.经过GUS基因组织化学染色检测、PCR检测以及Southern blot杂交分析,证实了叶片衰老抑制基因PSAG12-ipt已经整合到水稻基因组中.
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| 关 键 词： | 叶片衰老抑制基因PSAG12-ipt 基因枪 籼型不育系中A |
| 文章编号： | 0529-6579(2005)02-0078-04 |
| 修稿时间： | 2004年4月28日 |
Studies of the Leaf-specific Senescence-inhibitor Gene (PSAG12-ipt) Introduced into Zhong A (Cytoplasmic Male Sterile Indica) |
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| WANG Ya-qin,LIANG Cheng-ye.Studies of the Leaf-specific Senescence-inhibitor Gene (PSAG12-ipt) Introduced into Zhong A (Cytoplasmic Male Sterile Indica)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2005,44(2):78-81. |
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| Authors: | WANG Ya-qin LIANG Cheng-ye |
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| Abstract: | pSG516(ipt) and pHQ9(hpt and gus)were co-transferred into Zhong A (cytoplasmic male sterile indica) genomic DNA by the particle bombardment of Biolistic PSD-1000/He Particle Delivery System (BIO-RAD) with the plasmid DNA mixed to Gold Particle. Ninety-eight independent lines were obtained by selecting for hygromycin resistance. Results of GUS, PCR and Southern blot demonstrated that the leaf-specific senescence-inhibitor gene (P_(SAG12-ipt)) has been integrated into rice genome. |
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| Keywords: | leaf-specific senescence-inhibitor gene (P_(SAG12-ipt)) particle bombardment Zhong A (cytoplasmic male sterile indica) |
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