Transfilter associations of nonleguminous plants with rhizobia: Channelling of N2 fixed by rhizobia into the nitrogen-metabolism ofPortulaca grandiflora callus tissues

Summary In transfilter associations ofPortulaca grandiflora callus tissues withRhizobium sp. cowpea 32H1 the nitrogenium fixed by the bacteria was channelled into the normal pathway of nitrogen metabolism of higher plants. In associations kept in an¹⁵N₂ containing atmosphere 10% of the¹⁵N taken up into the plant cells was incorporated intoPortulaca proteins. The results demonstrate that cells of nonleguminous plants are profiting from the quasi-symbiotic situation.