| 高效液相色谱法检测人尿中维生素B2的含量 |
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| 引用本文： | 赖谷仙,俞英,潘颖瑜,杨海玉,郑秀丽.高效液相色谱法检测人尿中维生素B2的含量[J].华南师范大学学报(自然科学版),2009,1(1):67. |
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| 作者姓名： | 赖谷仙 俞英 潘颖瑜 杨海玉 郑秀丽 |
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| 作者单位： | 1.华南师范大学化学与环境学院 华南师范大学 |
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| 摘 要： | 摘要:研究了维生素B2经人体代谢后排放到尿样中的情况.服V B2片剂50mg，收集服药后1、2、4、6、8h后的尿样.服药尿样经固相萃取后，采用高效液相色谱法检测服用后尿样中维生素B2的含量.以乙腈/醋酸胺缓冲液(0.01mol／L)为流动相，检测波长267nm.该法具有样品预处理简单、灵敏度高、分析时间短等优点，成功应用于人体尿样中维生素B2的直接测定，加标回收率在91.2%~96.7%之间。
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| 关 键 词： | 维生素B2 代谢 固相萃取 高效液相色谱 |
| 收稿时间： | 2007-12-03 |
DETERMINATION OF VITAMIN B_2 IN HUMAN URINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| LAI Gu-xian,YU ying,PAN Ying-yu,YANG Hai-yu,ZHENG Xiu-li.DETERMINATION OF VITAMIN B_2 IN HUMAN URINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Journal of South China Normal University(Natural Science Edition),2009,1(1):67. |
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| Authors: | LAI Gu-xian YU ying PAN Ying-yu YANG Hai-yu ZHENG Xiu-li |
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| Institution: | School of Chemistry and Environment;South China Normal University;Guangzhou;510631 |
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| Abstract: | The excretion of vitamin B2 in human urine after oral administration of 50 mg vitamin B2 was studied.Urine samples in 1,2,4,6,8 hours were collected and extracted by solid-phase extraction.A HPLC method was established for determination of vitamin B2 in human urine with mobil phase of acetonitrile/ammonium buffer(0.01 mol/L).The compounds was detected directly by ultraviolet detector at 267 nm.This method is simply rapid,sensitive and cheap for directly determining vitamin B2 in human urine with the recover... |
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| Keywords: | vitamin B2 metabolite solid-phase extraction HPLC |
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