| 点青霉高产葡萄糖氧化酶菌株的诱变选育 |
| |
| 引用本文： | 胡常英,刘丽娜,王云鹏,马晓冬. 点青霉高产葡萄糖氧化酶菌株的诱变选育[J]. 河北省科学院学报, 2006, 23(3): 11-15 |
| |
| 作者姓名： | 胡常英 刘丽娜 王云鹏 马晓冬 |
| |
| 作者单位： | 河北省微生物研究所,河北,保定,071051;河北省微生物研究所,河北,保定,071051;河北省微生物研究所,河北,保定,071051;河北省微生物研究所,河北,保定,071051 |
| |
| 摘 要： | 以产葡萄糖氧化酶的点青霉(Penicilium notatum)No.8312为出发菌株,经紫外线诱变处理,对单菌落进行摇瓶选育,在193个突变株中初筛选定3株.进一步对培养基和发酵条件优化试验,复筛得到了一株(No.T111)高产葡萄糖氧化酶的菌株,连续12次对其传代试验,该突变株遗传性状稳定,摇瓶产酶水平达到55.7U/mL,比出发株(36.2U/mL)提高了53.9%.
|
| 关 键 词： | 点青霉 紫外线诱变 葡萄糖氧化酶 酶活 |
| 文章编号： | 1001-9383(2006)03-0011-05 |
| 修稿时间： | 2006-04-05 |
Mutation breeding of high glucose oxidase activity strains from penicillium notatum |
| |
| HU Chang-ying,LIU Li-na,WANG Yun-peng,MA Xiao-dong. Mutation breeding of high glucose oxidase activity strains from penicillium notatum[J]. Journal of The Hebei Academy of Sciences, 2006, 23(3): 11-15 |
| |
| Authors: | HU Chang-ying LIU Li-na WANG Yun-peng MA Xiao-dong |
| |
| Abstract: | As an original strain,Penicillium notatum No.8312 was mutated by UV mutagenesis.Three strains were obtained from 193 strains mutants.The seprarte single colony was inoculated in the shake flask experiments.Basing on the optimal proportion of culture medium and parameter of fermentation,one strain(No.T 111) with high yield glucose oxidase was obtained from mutants by screening.During twelve times continuous transfer culture,the result showed that the glucose oxidase concentration is 55.7U/ml in the shake flaske experiments,increasing 53.9% compared with strain No.8312(36.2 U/ml). |
| |
| Keywords: | Penicillium notatum UV treatment Glucose oxidase Glucose oxidase activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
