| 盐碱胁迫对不同苜蓿品种过氧化物同工酶的影响 |
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| 引用本文： | 李雪婷,李波,丁文超,石善亮,侯亚欣,李坤,金晨阳,吕艳玲.盐碱胁迫对不同苜蓿品种过氧化物同工酶的影响[J].高师理科学刊,2014(3):74-76. |
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| 作者姓名： | 李雪婷 李波 丁文超 石善亮 侯亚欣 李坤 金晨阳 吕艳玲 |
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| 作者单位： | 齐齐哈尔大学生命科学与农林学院; |
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| 基金项目： | 齐齐哈尔大学研究生创新科研项目(YJSCX2013-025X);黑龙江省大学生创新创业训练计划项目(201310232028);黑龙江省教育厅科学技术研究项目(12521610) |
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| 摘 要： | 为研究盐碱环境对苜蓿过氧化物同工酶的影响,以14个不同品种的苜蓿为研究对象,对其幼苗进行复合盐碱胁迫,采用聚丙烯凝胶电泳技术对过氧化物同工酶进行电泳分析.结果表明,苜蓿品种间的酶带数有所不同,过氧化物同工酶最多有9条,最少有4条,复合盐碱胁迫后差异性带主要出现在C区,酶活性弱,A区和B区无明显差异带.
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| 关 键 词： | 苜蓿 POD同工酶 盐碱胁迫 迁移率 |
Effect of PEG stress on POD isoenzymes in 14 Alfalfa varieties |
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| Institution: | LI Xue-ting;LI Bo;DING Wen-chao;SHI Shan-liang;HOU Ya-xin;LI Kun;JIN Chen-yang;Lü Yan-ling(School of Life Science and Agriculture and Forestry,Qiqihar University; Qiqihar 161006, China) |
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| Abstract: | To study the POD isoenzymes among 14 Alfalfa varieties of salt and alkali stress, the POD isoenzymes in the young leaves of 14 Alfalfa varieties were analyzed using the vertical stab polyacrylamide gel electrophoresis technology. The results showed that the Alfalfa varieties POD isozyme band number in 14 Alfalfa varieties was different, with up to 9, at least 4. CAT isozyme of different Alfalfa varieties, in the A area of the enzyme with no change, strong activity. The C area of the enzyme was new enzyme band, but the activity is weak, with little change in A and B district of enzyme. |
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| Keywords: | Alfalfa POD isoenzymes salt and alkali stress mobility |
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