| 促进剂对枯草芽胞杆菌CICC20958产肌苷的影响 |
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| 引用本文： | 游洪燕,杨哲,肖安风,倪辉,蔡慧农,杨秋明. 促进剂对枯草芽胞杆菌CICC20958产肌苷的影响[J]. 集美大学学报(自然科学版), 2014, 0(2): 107-112 |
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| 作者姓名： | 游洪燕 杨哲 肖安风 倪辉 蔡慧农 杨秋明 |
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| 作者单位： | 集美大学生物工程学院;福建省食品微生物与酶工程重点实验室;福建省高校食品微生物与酶工程技术研究中心;厦门市食品生物工程技术研究中心; |
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| 基金项目： | 福建省自然科学基金资助项目(2011J01225);集美大学中青年创新团队专项基金项目(2010A006) |
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| 摘 要： | 根据肌苷的代谢调控机理,选择柠檬酸钠、NaF、CaCl2、次黄嘌呤、MnSO45种肌苷促进剂,考察它们的不同添加量对枯草芽胞杆菌(Bacillus subtilis,简称Bs)CICC20958菌株产肌苷的影响,采用均匀设计法得到最佳组合添加方案.结果表明,在促进剂柠檬酸钠、NaF、CaCl2、次黄嘌呤、MnSO4质量浓度分别为7.9、0.001、0.032、2.1、0.2 g/L的条件下发酵,菌株Bs CICC20958的肌苷产量达到7.81 g/L,比不添加促进剂实验组(对照组)的肌苷产量提高了5.38倍.
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| 关 键 词： | 枯草芽胞杆菌 CICC20958 肌苷 促进剂 均匀设计试验 |
Effect of Accelerators on Inosine Production of Bacillus subtilis CICC20958 |
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| YOU Hong-yan,YANG Zhe,XIAO An-feng,NI Hui,CAI Hui-nong,YANG Qiu-ming. Effect of Accelerators on Inosine Production of Bacillus subtilis CICC20958[J]. the Editorial Board of Jimei University(Natural Science), 2014, 0(2): 107-112 |
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| Authors: | YOU Hong-yan YANG Zhe XIAO An-feng NI Hui CAI Hui-nong YANG Qiu-ming |
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| Affiliation: | 1. College of Biological Engineering, Jimei University, Xiamen 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; 3. Research Center of Food Microbiology and Enzyme Engineering Technology of Fujian Province, Xiamen 361021, China; 4. Research Center of Food Bioteehnology of Xiamen City, Xiamen 361021, China) |
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| Abstract: | According to the regulating mechanism of inosine synthesis, some kinds of effective additives （sodium citrate, NaF, CaC12, hypoxanthinc and MnSO4 ） were added in Bacillus subtilis CICC20958. Then, the best composition of accelerators was obtained by uniform design. The results showed that optimized combination increased inosine production to 7.81 g/L in the presence of 7.9 g/L sodium citrate, O. 001 g/L NaF, 0. 032 g/L CaC12, 2. 1 g/L hypoxanthine and 0. 2 g/L MnSO4. The yield of inosine was 5.38 times higher than that of the control group. |
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| Keywords: | Bacillus subtilis CICC20958 inosine accelerator uniform design |
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