LY333531对高糖高脂下系膜细胞分泌细胞外基质的影响

探讨PKCβ抑制剂(LY333531)对高糖高脂环境下系膜细胞分泌细胞外基质(ECM)的影响,单独培养人肾小球系膜细胞,实验分为对照组、LY333531组、高糖高脂组、高糖高脂+LY333531组.首先采用qRTPCR检测PKCβI的表达,然后通过ELISA法检测细胞上清液Col IV、Fn的含量.结果显示,高糖高脂组相较于对照组,PKCβI mRNA的表达水平明显增加(P0.05),LY333531可以明显抑制PKCβI的表达(P0.05);高糖高脂组相较于对照组,细胞上清液Col IV、Fn含量明显增加(P0.05),而这种作用可被LY333531所抑制(P0.05).表明高糖高脂可通过促进系膜细胞PKCβI表达从而诱导ECM的分泌,增加肾纤维化发生的风险,而LY333531可明显抑制这种作用.

Effect of LY333531 on the expression of extracellular matrix in mesangial cells exposed to high glucose and lysophosphatidylcholine

To investigate the effect of PKCβinhibitor (LY333531) on extracellular matrix (ECM ) of masangial cells in high glucose ( HG ) and lysophosphatidylcholine (LPC ) environment ,human mesangial cells (HMCs) were cultured alone and divided into 4 groups :control ;LY333531 ;HG and LPC(HG + LPC);HG ,LPC and LY333531(HG + LPC +LY333531) .First ,we used the method of quantitative real‐time PCR(qRT‐PCR) to detect the expression of PKCβI;then ,the levels of collagen IV and fibronectin were detected by enzyme linked immunosorbent assay (ELISA) in the supernatant .These results suggested that the mRNA expression of PKCβI in the group of HG+LPC was higher than the control group (P< 0 .05) ,and the effect could be obviously inhibited by LY333531 (P< 0 .05) . Meanwhile ,compared with the control group ,the level of collagen IV and fibronectin in the supernatant was significantly increased in the group of HG +LPC (P <0 .05) ,and the effect could be also inhibited by LY333531 notably (P<0 .05) .Therefore ,we could conclude that the HG+LPC can aggrandize the secretion of ECM by increasing the expression of PKCβI in mesangial cells , which can increase the risk of renal fibrosis , however , LY333531 can weaken the effect obviously .