Complete
nucleotide sequence and genomic organization of Periplaneta fuliginosa densonucleosis
virus |
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作者单位: | College of Life Science,Wuhan University,Wuhan 430072,China
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摘 要: |
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收稿时间: | 1 March 2000 |
Complete nucleotide sequence and genomic organization ofPeriplaneta fuliginosa densonucleosis virus |
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Authors: | Haitao Guo Jiamin Zhang Yuanyang Hu |
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Institution: | (1) College of Life Science, Wuhan University, 430072 Wuhan, China |
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Abstract: | We have cloned the replicative form of thePeriplaneta fuliginosa densonucleosis virus (PfDNV) genome and determined its complete sequence. The sequence has 5 454 nucleotides (nt), the genome
consists of an internal unique sequence flanked by inverted terminal repeats (201 nt). The first 122 nt at the 5′ end and
the terminal 122 nt at the 3′ end of both plus and minus strands can fold into a typical hairpin structure. The genome contains
seven major open reading frames (ORFs). The plus strand has 4 ORFs occupying the 5′ half of the plus strand, whereas the others
span the 5′ half of the minus strand. Two potential promoters were found at map units (m.u.) 3 and 97. Computer analysis of
sequence homologies with other parvoviruses suggests that the plus strand ofPf DNV encodes very likely the nonstructural proteins and the minus strand probably encodes the structural proteins. |
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Keywords: | Periplaneta fuliginosa densovirus nucleotide sequence genomic organization |
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